PMID: 6400029Aug 1, 1984Paper

Electron microscopy and image processing applied to the study of protein structure and protein-protein interactions

Journal of Ultrastructure Research
U AebiP R Smith

Abstract

We review the application of electron microscopy and image processing at the molecular level to an ever increasing range of biological specimens. Although recent advances have been due in part to development of more sophisticated instrumentation and/or processing algorithms, widespread application of the well-known techniques of image enhancement and structure reconstruction has depended on new strategies of in vitro crystallization and polymerization, some of which are outlined here. We also discuss the use of stoichiometric labeling and/or "cocrystallization" in identifying the different subunits in multisubunit complexes and in studying protein-protein interactions.

References

Jan 1, 1978·Ultramicroscopy·J FrankT S Baker
Dec 15, 1979·Journal of Molecular Biology·T E Wellems, R Josephs
Apr 1, 1977·Journal of Ultrastructure Research·J KistlerE Kellenberger
Oct 25, 1979·Journal of Molecular Biology·S D FullerR Henderson
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T G FreyF A Eiserling
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·U AebiM K Showe
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W Baumeister, O Kübler
Feb 1, 1977·The Journal of Cell Biology·A C StevenJ P Rosenbusch
Dec 5, 1979·Journal of Molecular Biology·M W ClarkJ A Lake
Mar 1, 1979·Journal of Ultrastructure Research·R M GlaeserD Grano
Feb 1, 1977·FEBS Letters·H G MannherzR Leverman
Oct 20, 1978·Science·J Dubochet, M Noll
Dec 14, 1978·Nature·C G dos Remedios, M J Dickens
Feb 25, 1977·Journal of Molecular Biology·N M Tooney, C Cohen
Aug 25, 1977·Journal of Molecular Biology·R AschaffenburgM P Esnouf
Oct 1, 1977·Journal of Ultrastructure Research·P R Smith, J Kistler
Jun 1, 1976·Journal of Ultrastructure Research·K A Taylor, R M Glaeser
Aug 15, 1976·Journal of Molecular Biology·L CarlssonS Lövgren
Jan 1, 1976·Journal of Supramolecular Structure·U AebiP R Smith
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J P Langmore, J C Wooley
Jan 1, 1975·Annual Review of Pharmacology·J B Cohen, J P Changeux
May 25, 1975·Journal of Molecular Biology·P N Unwin, R Henderson
Sep 5, 1975·Journal of Molecular Biology·R Josephs
May 11, 1973·Molecular and Cellular Biochemistry·L I RebhunJ Larner

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Citations

Aug 1, 1993·Molecular Immunology·R G DiScipio
Mar 13, 2001·Journal of Structural Biology·E HahnU Aebi
Aug 1, 1988·Journal of Electron Microscopy Technique
Aug 1, 1988·Journal of Electron Microscopy Technique·M Stewart
Mar 5, 1989·Journal of Molecular Biology·R NewmanM J Crumpton
Aug 18, 2009·Pulmonary Pharmacology & Therapeutics·Bruno T MenegaliRicardo A Pinho

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