Electron microscopy of chromatin

Methods : a Companion to Methods in Enzymology
C L Woodcock, R A Horowitz

Abstract

Electron microscopy, with its ability to image DNA and nucleosomes, can provide a key visual link in the understanding of chromatin conformation. We discuss applications of EM to current chromatin research with emphasis on strategies that eliminate many of the potential problems associated with conventional EM preparative techniques. Cryo-electron microscopy (cryo-EM) of isolated chromatin, whereby samples are imaged "in solution" in thin vitrified films, is considered in detail, with emphasis on the recovery of three-dimensional information and on its application to linker DNA conformation and to salt-induced compaction. Factors that currently limit the technique, and the prospects of overcoming them, are also considered.

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·W KellerH Zentgraf
Jan 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R A Horowitz, C L Woodcock
Oct 1, 1992·Ultramicroscopy·A J KosterD A Agard
Feb 28, 1992·Biochimica Et Biophysica Acta·F Thoma
Aug 1, 1991·Journal of Structural Biology·I DustinE Egelman
Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·R T Simpson
Jan 1, 1990·Electron Microscopy Reviews·I RaskaL Salamin-Michel
Dec 1, 1990·Journal of Electron Microscopy Technique·M CyrklaffJ Dubochet
Feb 20, 1990·Journal of Molecular Biology·D J Clark, T Kimura
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Feb 20, 1986·Journal of Molecular Biology·J AllanC Crane-Robinson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S E Gerchman, V Ramakrishnan
Feb 20, 1986·Nature·M Stewart, G Vigers
Jan 25, 1974·Science·A L Olins, D E Olins
Jan 1, 1972·Acta Endocrinologica. Supplementum·O L Miller, A H Bakken
May 23, 1969·Science·O L Miller, B R Beatty
Jul 1, 1984·The Journal of Cell Biology·C L WoodcockJ B Rattner
May 1, 1995·Journal of Structural Biology·P FurrerA Prunell
Apr 14, 1995·The Journal of Biological Chemistry·K van Holde, J Zlatanova
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·S PenningsE M Bradbury
Apr 1, 1994·Current Opinion in Genetics & Development·A P Wolffe
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C L WoodcockN Whitaker
Mar 22, 1996·Journal of Molecular Biology·A HamicheA Prunell
Jan 1, 1996·Critical Reviews in Eukaryotic Gene Expression·T M Fletcher, J C Hansen
May 1, 1997·Methods : a Companion to Methods in Enzymology·J C HansenT M Fletcher
Jul 1, 1995·Trends in Cell Biology·C L Woodcock, R A Horowitz

❮ Previous
Next ❯

Citations

Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Emilios K DimitriadisYamini Dalal
Jun 12, 2010·PMC Biophysics·Philipp M Diesinger, Dieter W Heermann
May 20, 2014·Biophysics Reviews·Yuri L Lyubchenko
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BednarC L Woodcock
Apr 27, 2001·European Journal of Biochemistry·Y YoshikawaK Yoshikawa
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Joan-Ramon Daban
Oct 6, 1999·The Journal of Cell Biology·P J VerschureR van Driel
Nov 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G LattanziG Mazzotti
Feb 20, 1999·The Journal of Biological Chemistry·S A GrigoryevC L Woodcock
Sep 21, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·K-K Kunze, R R Netz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.