PMID: 2120705Oct 1, 1990Paper

Electron microscopy of human factor V and factor VIII: correlation of morphology with domain structure and localization of factor V activation fragments

Proceedings of the National Academy of Sciences of the United States of America
W E FowlerV J Marder

Abstract

Clotting factor V and factor VIII are each represented by the domain structure A1-A2-B-A3-C1-C2 and share 40% sequence homology in the A and C domains. Rotary-shadowed samples of human factor V and factor VIII were examined in the electron microscope. Single-chain factor V molecules exhibited a globular "head" domain 12-14 nm in diameter. In addition, up to 25% of these molecules showed a rod-like "tail" of up to 50 nm. Glycerol-gradient centrifugation of factor V treated with thrombin partially resolved the factor Va heterodimer from a larger activation peptide of 150 kDa, as determined by gel electrophoresis. Electron microscopy of factor Va revealed globular molecules with several smaller appendicular structures but lacking the tails seen in factor V. Images of the 150-kDa activation peptide showed rod-like structures, similar in width to the tail of intact factor V and approximately 34 nm long. Rotary shadowing was also used to visualize factor VIII that had been fractionated into heterodimers containing heavy chains of distinct sizes. Each factor VIII preparation showed a globular structure approximately 14 nm in diameter, but the associated tails were observed much more frequently with factor VIII heterodimers containing ...Continue Reading

References

Oct 1, 1985·The Journal of Clinical Investigation·W E FowlerP A McKee
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J J TooleR J Kaufman
Jun 23, 1986·Biochimica Et Biophysica Acta·P J FayV J Marder
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J JennyK G Mann
Jan 29, 1988·Biochimica Et Biophysica Acta·P J Fay
Feb 1, 1967·Biopolymers·V BloomfieldK E Van Holde
Jun 1, 1983·Journal of Ultrastructure Research·W E Fowler, U Aebi
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A KatzmannK G Mann
Sep 20, 2007·The Journal of Biological Chemistry·Kenneth SegersGerry A F Nicolaes

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Citations

Jul 9, 1998·Protein Science : a Publication of the Protein Society·B O Villoutreix, B Dahlbäck
Jun 6, 2003·International Journal of Pharmaceutics·Wei WangDrew N Kelner
Jan 6, 1994·The New England Journal of Medicine·L W Hoyer
Jun 19, 2012·The Journal of Biological Chemistry·Mettine H A Bos, Rodney M Camire
Dec 24, 2013·Analytical and Bioanalytical Chemistry·Klaus BonazzaGernot Friedbacher
May 29, 2009·Haemophilia : the Official Journal of the World Federation of Hemophilia·S W Pipe
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·R Manjunatha Kini
May 24, 2015·Analytical and Bioanalytical Chemistry·Klaus BonazzaGernot Friedbacher
Aug 10, 2000·The Journal of Pharmacy and Pharmacology·A FatourosB Sjöström
May 15, 1994·European Journal of Biochemistry·N BihoreauM P Fontaine-Aupart
Sep 22, 2009·Journal of Thrombosis and Haemostasis : JTH·R M Camire, M H A Bos
Mar 28, 2007·The Journal of Biological Chemistry·Hua ZhuRodney M Camire
Sep 10, 2013·The Journal of Biological Chemistry·Matthew W BunceRodney M Camire
Jun 17, 1998·The Journal of Biological Chemistry·Y KojimaJ H Griffin
Dec 14, 1999·The Journal of Biological Chemistry·S S StoylovaA Holzenburg
Jan 24, 1997·The Journal of Biological Chemistry·K A Lapan, P J Fay
Jun 20, 1998·The Journal of Biological Chemistry·E ThorelliB Dahlbäck
Sep 20, 1996·The Journal of Biological Chemistry·K Sudhakar, P J Fay
Mar 9, 2021·Blood·Eliza A RubenEnrico Di Cera
Mar 28, 1995·Biochemistry·F G KellerW H Kane

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