PMID: 7026472Mar 1, 1981Paper

Electron microscopy of network structures in thermally-induced globular protein gels

International Journal of Peptide and Protein Research
A H ClarkA Suggett

Abstract

Thin sections of heat-set proteins gels formed from bovine serum albumin, insulin, lysozyme, ribonuclease, and alpha-chymotrypsin, have been studied by transmission electron microscopy. Micrographs have been interpreted as showing protein networks with strands between one and two times as thick as the native protein diameters. Considerable differences in the persistence characteristics, and frequencies of cross-linking, of the strands are observed, and there are variations in network homogeneity over long distances which correlate well with changes in gel opacity caused by alterations in pH and ionic strength. Evidence that artefacts are unlikely to have influenced these interpretations has been obtained in the BSA case in particular, by studying the aggregation process in solution, using alternative microscope approaches such as heavy-metal shadowing and negative staining. assuming that artefacts are absent, gel section micrographs have been simulated by a computer procedure, and the results suggest that, in most cases, the simplest interpretation of the microscope data is in terms of a "string of beads" model for the aggregation process, involving only moderately unfolded, and still globular, protein molecules. Other structur...Continue Reading

References

Aug 1, 1979·Angewandte Chemie·M G Bezrukov
Jan 1, 1977·Advances in Protein Chemistry·P JonathanA C Durham
Jan 1, 1974·Faraday Discussions of the Chemical Society·M P Tombs
Jan 1, 1973·Advances in Protein Chemistry·R F Doolittle
Jan 1, 1969·Quarterly Reviews of Biophysics·R E Stephens
Jun 20, 1972·Biochemistry·M J Burke, M A Rougvie
Nov 1, 1969·European Journal of Biochemistry·G H BeavenH G Davies
Oct 1, 1980·International Journal of Peptide and Protein Research·A H Clark, C D Tuffnell

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Citations

Oct 2, 2004·Protein Science : a Publication of the Protein Society·Peter B StathopulosElizabeth M Meiering
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Mireille WeijersRonald W Visschers
Jul 16, 2010·Journal of Food Science·Bongkosh VardhanabhutiE Allen Foegeding
Apr 28, 2010·Chemical Society Reviews·Congqi Yan, Darrin J Pochan
Jun 1, 2012·The Protein Journal·Amir ArastehAli Akbar Moosavi-Movahedi
Jul 23, 2013·Journal of Dairy Science·Hee-Dong WooSanghoon Ko
Mar 30, 2007·Journal of Microencapsulation·Sanghoon Ko, Sundaram Gunasekaran
Dec 1, 2009·Neurochemical Research·Mohammad R KhazaeiMehran Habibi-Rezaei
Jan 17, 2021·Cancers·Olivier BillantCécile Voisset
Apr 30, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Anne RenaultMichel Pézolet
Feb 14, 2004·Biophysical Chemistry·Valeria MilitelloMaurizio Leone
Jun 1, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Anne RenaultMichel Pézolet

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