PMID: 14414323Jun 1, 1960Paper

Electron microscopy of retinal photoreceptors. The use of chromation following formaldehyde fixation as a complementary technique to osmium tetroxide fixation

The Journal of Biophysical and Biochemical Cytology
A LASANSKY, E DE ROBERTIS

Abstract

The fine structure of the cone and rod outer segments of the toad was studied under the electron microscope after fixation in osmium tetroxide and fixation in formaldehyde followed by chromation. In the OsO(4)-fixed specimens, the rod outer segment appears to be built of a stack of lobulated flattened sacs, each of which is made of two membranes of about 40 A separated by an innerspace of about 30 A. The distance between the rod sacs is about 50 A. The sacs in the cone outer segment are originated by the folding of a continuous membrane. The thickness of the membranes and width of the spaces between the cone sacs is the same as in rod, but the sac innerspace is slightly narrower in the cone ( approximately 20 A). After fixation in formaldehyde and chromation, two different dense lines (l(1) and l(2)) separated by spaces of less density appear. One of the lines, l(1), has a thickness of 70 A and is less dense than the other, l(2), which is 30 A thick. The correlation of the patterns obtained with both fixatives is considered and two possible interpretations are given. The possibility that l(2) is related to a soluble phospholipid component is discussed. It is suggested that the outer segments have a paracrystallin organization s...Continue Reading

References

May 25, 1956·The Journal of Biophysical and Biochemical Cytology·E DE ROBERTIS, C M FRANCHI
Dec 1, 1956·Journal of Cellular Physiology·J J WOLKEN
Apr 1, 1958·Journal of Ultrastructure Research·F S SJOSTRAND, R F BAKER
Nov 25, 1958·The Journal of Biophysical and Biochemical Cytology·E DE ROBERTIS, A LASANSKY
Nov 12, 1959·Annals of the New York Academy of Sciences·G TOLLINM CALVIN
May 25, 1959·The Journal of Biophysical and Biochemical Cytology·W STOECKENIUS
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·K TOKUYASU, E YAMADA
Mar 1, 1952·The Anatomical Record·R D LILLIE
Mar 1, 1952·The Journal of Experimental Medicine·G E PALADE

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Citations

Apr 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J D Robertson
Jan 1, 1967·J R Microsc Soc·J R Casley-Smith
May 31, 2007·The Journal of Cell Biology·Stephan NickellKrzysztof Palczewski
Jan 4, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Kevin C ChanEd X Wu
Feb 1, 1964·Biological Reviews of the Cambridge Philosophical Society·M F MOODY
Feb 1, 1963·Journal of Ultrastructure Research·G M VILLEGAS, R VILLEGAS
Sep 6, 1961·Annals of the New York Academy of Sciences·J D ROBERTSON

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