Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat

The Journal of Nutritional Biochemistry
Ken-Ichiro MatsumotoKazutoyo Endo

Abstract

The free radical-reducing activity and the membrane fluidity of liver microsomes from selenium-deficient (SeD) rats were examined by means of electron paramagnetic resonance (EPR) spin label method using nitroxyl-labeled stearic acids. Our findings show that the membrane fluidity and lipid peroxidation levels in SeD rat liver microsome were relatively unchanged compared with normal rat. In contrast, SeD caused the induction of liver microsomal cytochrome P-450 activity. The nitroxyl spin probes are substrates for reduction-relating cytochrome P-450. Previous in vivo studies suggested that the total liver free radical reduction activity in SeD rat was decreased. In contrast, SeD caused the induction of liver microsomal cytochrome P-450 activity, and the reduction rate of nitroxyl radical existing at shallow depth in membrane was increased. Selenium-deficient rats experienced an increase in hydrogen peroxide (H2O2) due to a pronounced loss of glutathione peroxidase (GSH-Px) activity. This masked the overall reduction rate of the nitroxyl spin probe by reoxidation of the hydroxylamine form. Although the SeD condition caused induction of liver cytochrome P-450 and chronic increased H2O2, this did not result in oxidative liver damag...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Aug 15, 1989·Biochemical Pharmacology·A IannoneV Vannini
Jul 1, 1985·Archives of Biochemistry and Biophysics·K E Hill, R F Burk
May 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·M A BroderiusP H Weswig
Nov 1, 1980·Proceedings of the Society for Experimental Biology and Medicine·R A Sunde, W G Hoekstra
Jan 1, 1993·Archives of Biochemistry and Biophysics·Y MiuraA Hamada
Jan 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M EngelkeH A Diehl
May 18, 2000·Biochemical and Biophysical Research Communications·Y UedaK Endo
May 24, 2000·Biological & Pharmaceutical Bulletin·K MatsumotoH Utsumi
Nov 16, 2001·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·K MatsumotoK Endo
Apr 24, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Ken-ichiro MatsumotoKazutoyo Endo
Jun 29, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Ken-ichiro MatsumotoKazutoyo Endo
Sep 28, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Govindasamy IlangovanPeriannan Kuppusamy
Apr 12, 2003·Redox Report : Communications in Free Radical Research·Ken-ichiro MatsumotoKazutoyo Endo
May 31, 2003·Science·Gregory V KryukovVadim N Gladyshev
Nov 17, 2004·Free Radical Biology & Medicine·Iori UiKen-Ichiro Matsumoto

❮ Previous
Next ❯

Citations

Jul 27, 2007·The British Journal of Nutrition·Alessandra BordoniPier Luigi Biagi
Jul 6, 2005·Biological & Pharmaceutical Bulletin·Ken-ichiro MatsumotoKazutoyo Endo
Mar 1, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Pinar ErkekogluFiliz Hincal
May 26, 2007·Chemistry and Physics of Lipids·Yasunobu SakumaMasamichi Tsukada
Sep 29, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Ken-Ichiro MatsumotoKen-Ichi Yamada

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.