PMID: 7541503Jan 1, 1995Paper

Electron paramagnetic resonance investigations of nitrosyl complex formation during endotoxin tolerance

Life Sciences
W ChamulitratJ A Spitzer

Abstract

The prior administration of low dose endotoxin induces a state of hyporesponsiveness or tolerance to the lethal effects of endotoxin. It is generally accepted that macrophages are main cellular components in the development of tolerance, hence, nitric oxide (.NO) as one of the macrophage mediators may play a role in host defense mechanisms during tolerance. In this study, we utilized EPR spectroscopy to directly detect nitrosyl complexes as products of .NO in whole blood, livers and intestines of lipopolysaccharide (LPS)-tolerant rats. Male Sprague-Dawley rats were injected with a "low dose" LPS (0.5 mg/kg) 12-168 h prior to a "high dose" LPS (3 mg/kg), then sacrificed 6 h later. EPR signals of nitrosyl hemoprotein complexes were detected in specimens after high dose LPS. The post-LPS EPR signals of nitrosyl complexes from all samples were attenuated by a prior injection of low dose LPS. The signals of dinitrosyl-iron-dithiolate became apparent in samples from tolerant rats as signals of nitrosyl hemoprotein decreased. The maximal tolerance in terms of diminished .NO production was observed when low dose LPS was given 48-96 h prior to high dose LPS. Hemoglobin concentrations in the intestine used as biomarkers of hemorrhagic da...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A CarswellB Williamson
Feb 1, 1992·Archives of Surgery·P LeónJ M Daly
Sep 1, 1991·European Journal of Immunology·G F Evans, S H Zuckerman
Jan 1, 1990·Free Radical Research Communications·U WestenbergerO Trentz
Dec 1, 1990·British Journal of Pharmacology·I R HutchesonN K Boughton-Smith
Apr 1, 1990·The Journal of Surgical Research·T R BilliarR L Simmons
Jul 30, 1993·Biochemical and Biophysical Research Communications·A K NüsslerJ R Lancaster
Mar 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A P Bautista, J J Spitzer
Oct 1, 1994·The American Journal of Physiology·J GeiselP V Halushka
May 30, 1994·FEBS Letters·C S Lai, A M Komarov
Sep 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y HenryA Guissani
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Archer
Mar 15, 1993·Biochemical and Biophysical Research Communications·A RojasS Moncada

❮ Previous
Next ❯

Citations

Oct 10, 1996·European Journal of Pharmacology·E FujiiT Muraki
Sep 18, 1999·Free Radical Biology & Medicine·A MülschM A Foster
Apr 24, 2003·Critical Reviews in Toxicology·Lutz W D WeberAndreas Stampfl
Apr 1, 1997·Expert Opinion on Investigational Drugs·J P Hieble, R R Ruffolo
Jun 16, 1999·Nitric Oxide : Biology and Chemistry·Y KotakeL A Reinke
Jul 21, 1998·Annals of the New York Academy of Sciences·C Szabó

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.