Electron partitioning between the two branching quinol-oxidizing pathways in Acanthamoeba castellanii mitochondria during steady-state state 3 respiration.

The Journal of Biological Chemistry
Wieslawa JarmuszkiewiczFrancis E Sluse

Abstract

Amoeba mitochondria possess a respiratory chain with two quinol-oxidizing pathways: the cytochrome pathway and the cyanide-resistant alternative oxidase pathway. The ADP/O method, based on the non-phosphorylating property of alternative oxidase, was used to determine contributions of both pathways in overall state 3 respiration in the presence of GMP (an activator of the alternative oxidase in amoeba) and succinate as oxidizable substrate. This method involves pair measurements of ADP/O ratios plus and minus benzohydroxamate (an inhibitor of the alternative oxidase). The requirements of the method are listed and verified. When overall state 3 respiration was decreased by increasing concentrations of n-butyl malonate (a non-penetrating inhibitor of succinate uptake), the quinone reduction level declined. At the same time, the alternative pathway contribution decreased sharply and became negligible when quinone redox state was lower than 50%, whereas the cytochrome pathway contribution first increased and then passed through a maximum at a quinone redox state of 58% and sharply decreased at a lower level of quinone reduction. This study is the first attempt to examine the steady-state kinetics of the two quinol-oxidizing pathways...Continue Reading

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Citations

Apr 17, 2007·Journal of Bioenergetics and Biomembranes·Malgorzata CzarnaWieslawa Jarmuszkiewicz
Aug 6, 2010·Journal of Bioenergetics and Biomembranes·Malgorzata B SlocinskaYuru Deng
Apr 28, 2011·Journal of Bioenergetics and Biomembranes·Andrzej Woyda-PloszczycaWieslawa Jarmuszkiewicz
Sep 23, 2003·Infection and Immunity·Shamima AkhterJohn R Perfect
May 28, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nina Antos-Krzeminska, Wieslawa Jarmuszkiewicz
May 16, 2014·Parasitology·Natalia MalloJosé M Leiro
Mar 6, 2007·Journal of Bioenergetics and Biomembranes·Aleksandra SwidaWieslawa Jarmuszkiewicz
Jan 29, 2011·Journal of Bioenergetics and Biomembranes·Vicente de Paulo MartinsSérgio Akira Uyemura
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F E Sluse, W Jarmuszkiewicz
Mar 17, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F E Sluse, W Jarmuszkiewicz
Jun 14, 2005·Biochimica Et Biophysica Acta·Wieslawa JarmuszkiewiczFrancis E Sluse
Sep 29, 2004·Biochimica Et Biophysica Acta·Oscar JuárezJuan Pablo Pardo
Jan 20, 2010·FEBS Letters·Wieslawa JarmuszkiewiczIzabela Koszela-Piotrowska
Apr 14, 2007·The Journal of Biological Chemistry·Anna KicinskaWieslawa Jarmuszkiewicz
Sep 13, 2006·The FEBS Journal·Oscar JuárezJuan P Pardo
Aug 7, 1999·The Journal of Biological Chemistry·W JarmuszkiewiczF E Sluse
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Apr 16, 2009·Biochimica Et Biophysica Acta·Andrzej M Woyda-PloszczycaWieslawa Jarmuszkiewicz
May 26, 2009·International Journal for Parasitology·Fiona L HenriquezCraig W Roberts
Dec 23, 2009·Biochimica Et Biophysica Acta·Wieslawa JarmuszkiewiczFrancis E Sluse

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