PMID: 7370783May 12, 1980Paper

Electron probe microanalysis of isolated brain capillaries poisoned with lead

Brain Research
E K SilbergeldG W Goldstein

Abstract

The blood-brain barrier has been proposed as an important site for the toxic action of lead in the central nervous system. To investigate this, capillary endothelial cells were isolated from rat cortex and exposed to lead in vitro. Tissue suspensions were then prepared for electron microscopy and X-ray microprobe analysis. In cells exposed in vitro to lead, electron-dense deposits were observed within mitochondria. With X-ray analysis, it was determined that these intramitochondrial deposits contained lead in a non-crystalline matrix. Also, lead appeared to be accumulated in the same intramitochondrial areas as calcium. The results suggest that lead is preferentially sequestered in mitochondria of capillary endothelial cells. Further, this selective localization may be associated with lead-induced disruptions in intracellular calcium metabolism and transepithelial transport processes.

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Citations

Jul 1, 1981·Archives of Toxicology·A W Klein, T R Koch
Oct 1, 1982·Toxicology and Applied Pharmacology·J G PoundsR L Kodell
Sep 1, 1990·Brain Research. Developmental Brain Research·M J Engle, J J Volpe
Oct 14, 2003·Toxicology and Applied Pharmacology·Wei ZhengJean-Francois Ghersi-Egea
Aug 1, 1985·Journal of Neurology, Neurosurgery, and Psychiatry·M D Benson, J Price
Nov 1, 1987·Environmental Health Perspectives·T W Clarkson
Feb 8, 2000·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·B SzynakaA Augustynowicz
Dec 13, 2002·Brain : a Journal of Neurology·Theodore I Lidsky, Jay S Schneider
Jun 30, 1986·Toxicology and Applied Pharmacology·K MaxwellP A Cancilla
Mar 1, 1984·The American Journal of Physiology·J B Pickett, J C Bornstein

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