PMID: 6400028Aug 1, 1984Paper

Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed-beam electron microscope

Journal of Ultrastructure Research
F P Ottensmeyer

Abstract

A new imaging modality in electron microscopy uses energy filtration to produce micrographs with elastically scattered electrons or with electrons that have lost a specific, often characteristic amount of energy in interacting with the specimen. No deleterious effects on microscope performance are encountered. Instead, microanalysis of specimens is made possible with a spatial resolution of 3 to 5 A and a sensitivity of detection of 2 X 10(-21) g corresponding to about 50 atoms of phosphorus. Elements detected range from hydrogen (Z = 1) to uranium (Z = 92). Examples of elemental mapping show membrane structure, DNA within nucleosomes, and RNA within ribosomal particles.

References

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Citations

Oct 1, 1991·Journal of Biomedical Materials Research·M J FiliaggiR M Pilliar
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