Electron tomography and immunonanogold electron microscopy for investigating intracellular trafficking and secretion in human eosinophils.

Journal of Cellular and Molecular Medicine
Rossana C N MeloP F Weller

Abstract

Electron tomography (ET) has increasingly been used to understand the complexity of membrane systems and protein-trafficking events. By ET and immunonanogold electron microscopy, we recently defined a route for vesicular transport and release of granule-stored products from within activated human eosinophils, cells specialized in the secretion of numerous cytokines and other proteins during inflammatory responses. Here, we highlight these techniques as important tools to unveil a distinct eosinophil vesicular system and secretory pathway.

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Citations

Sep 30, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Rossana C N MeloPeter F Weller
Mar 25, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rossana C N MeloPeter F Weller
Nov 12, 2014·Frontiers in Immunology·Lisa A SpencerPeter F Weller
Nov 2, 2014·Journal of Clinical Pathology·Kelley E CapocelliEric P Wartchow
Aug 27, 2016·Experimental Cell Research·Rossana C N Melo, Peter F Weller
Sep 12, 2017·Nature Reviews. Immunology·Peter F Weller, Lisa A Spencer
Nov 12, 2016·Frontiers in Cell and Developmental Biology·Praveen AkuthotaRossana C N Melo
May 12, 2018·Journal of Leukocyte Biology·Rossana C N Melo, Peter F Weller

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Methods Mentioned

BETA
electron tomography
transmission electron microscopy

Software Mentioned

Xplore 3D
IMOD

Related Concepts

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