Electron tomography of ice-embedded prokaryotic cells

Biophysical Journal
R GrimmW Baumeister

Abstract

Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a resolution of 20-40 nm from tilt series comprising 50-140 images. The dose was kept within tolerable limits. A resolution of 20 nm allowed visualization of the individual stalks of the S-layer of Pyrobaculum aerophilum cells, which had undergone partial lysis, in three dimensions. The attainable resolution for low-dose electron tomography under different experimental conditions was theoretically investigated in terms of the specimen thickness. To obtain 2-nm resolution at 120 kV (300 kV), the specimen must not be thicker than 100 nm (150 nm). For a resolution of 10 nm, the maximum thickness is 450 nm (700 nm). An accelerating voltage of 300 kV is advantageous, mainly for specimens thicker than 100 nm. Experimental investigations so far have resulted in a resolution that is worse by a factor of 2-5 as compared to theory.

References

Oct 1, 1992·Ultramicroscopy·A J KosterD A Agard
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C SchleperW Zillig
Jul 1, 1992·Journal of Microscopy·D P BarnardB F McEwen
Jan 1, 1989·Canadian Journal of Microbiology·W BaumeisterB M Phipps
Dec 1, 1988·Journal of Virological Methods·Y Hosaka, T Watabe
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Jul 1, 1995·Ultramicroscopy·O L KrivanekB Kraus
Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
Jan 1, 1997·Biophysical Journal·R GrimmW Baumeister
Jul 1, 1997·Journal of Microscopy·S W HellH T van der Voort

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Citations

Dec 18, 2001·Journal of Neuroscience Research·G A PerkinsM H Ellisman
Oct 31, 2008·Extremophiles : Life Under Extreme Conditions·Albert F EllenArnold J M Driessen
Jun 24, 2009·Photosynthesis Research·Martin F Hohmann-Marriott, Robert W Roberson
Dec 4, 2003·Journal of Structural Biology·José Jesús Fernández, Sam Li
Dec 5, 2002·Current Opinion in Structural Biology·Wolfgang Baumeister
Jun 22, 2002·Trends in Biochemical Sciences·Matthew K Higgins, Harvey T McMahon
Aug 6, 2002·Journal of Structural Biology·S Van AertA van den Bos
Aug 6, 2002·Journal of Structural Biology·Joachim FrankCarmen A Mannella
Aug 6, 2002·Journal of Structural Biology·Bruce F McEwenCarmen Mannella
Aug 6, 2002·Journal of Structural Biology·Chyong-Ere HsiehCarmen A Mannella
May 8, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Christian KübelJohn Bradley
Apr 5, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Fernanda AbreuUlysses Lins
Aug 12, 1998·Nature Structural Biology·C Jacobsen, J Kirz
Feb 17, 2006·Nature Reviews. Molecular Cell Biology·Stephan NickellWolfgang Baumeister
Oct 11, 2012·Nature Reviews. Molecular Cell Biology·Karen FridmanOhad Medalia
Aug 12, 2009·Nature Reviews. Microbiology·Jacqueline L S Milne, Sriram Subramaniam
Oct 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·David ShapiroDavid Sayre
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lu GanGrant J Jensen
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John A BerrimanPeter B Rosenthal
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·J BohmW Baumeister
Dec 31, 2003·Molecular Biology of the Cell·Carolyn A Larabell, Mark A Le Gros
Dec 17, 2008·Journal of Bacteriology·Arnon LieberOhad Medalia
Jun 15, 2005·Annual Review of Biochemistry·Vladan LucićWolfgang Baumeister
Jul 31, 2010·Archaea : an International Microbiological Journal·Albert F EllenSonja-Verena Albers
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Jacques Izard
Nov 24, 2004·Biological Chemistry·Wolfgang Baumeister
Sep 7, 2013·Journal of Extracellular Vesicles·Dae-Kyum KimYong Song Gho
Oct 13, 2015·Journal of Molecular Biology·Shoh AsanoWolfgang Baumeister
Dec 1, 2009·Ultramicroscopy·J D Woodward, B T Sewell
Aug 12, 2009·Seminars in Cell & Developmental Biology·Montserrat Bárcena, Abraham J Koster
Mar 1, 2016·Nature Reviews. Microbiology·Catherine M OikonomouYi-Wei Chang
Apr 3, 2007·Current Opinion in Structural Biology·Grant J Jensen, Ariane Briegel
May 1, 2008·Journal of Microscopy·C Hagen, K Grünewald
Feb 28, 2006·Biology of the Cell·Cédric MessaoudiSergio Marco
Aug 13, 2010·Journal of Microscopy·M A AronovaR D Leapman
Jun 8, 2012·Journal of Microscopy·Christoph A DiebolderRoman I Koning

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