Electron tomography of late stages of FcRn-mediated antibody transcytosis in neonatal rat small intestine.

Molecular Biology of the Cell
Mark S LadinskyP J Bjorkman

Abstract

The neonatal Fc receptor (FcRn) transports maternal immunoglobulin (IgG) across epithelia to confer passive immunity to mammalian young. In newborn rodents, FcRn transcytoses IgG from ingested milk across the intestinal epithelium for release into the bloodstream. We used electron tomography to examine FcRn transport of Nanogold-labeled Fc (Au-Fc) in neonatal rat jejunum, focusing on later aspects of transport by chasing Au-Fc before fixation. We observed pools of Au-Fc in dilated regions of the lateral intercellular space (LIS), likely representing exit sites where Au-Fc accumulates en route to the blood. Before weaning, the jejunum functions primarily in IgG transport and exhibits unusual properties: clathrin-rich regions near/at the basolateral LIS and multivesicular bodies (MVBs) expressing early endosomal markers. To address whether these features are related to IgG transport, we examined LIS and endocytic/transcytotic structures from neonatal and weaned animals. Weaned samples showed less LIS-associated clathrin. MVBs labeled with late endosomal/lysosomal markers were smaller than their neonatal counterparts but contained 10 times more internal compartments. These results are consistent with hypotheses that clathrin-rich ...Continue Reading

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Citations

Aug 8, 2013·PloS One·Sudhasri MohantyClark L Anderson
Nov 16, 2013·Pediatric Research·Tonya L WardIllimar Altosaar
Nov 29, 2013·Journal of Pharmaceutical Sciences·Benjamin Wu, Yu-Nien Sun
Mar 15, 2015·Molecular Immunology·E Sally WardRaimund J Ober
Apr 21, 2016·Drug Discovery Today·Weiwei FanYong Gan
Nov 5, 2014·Frontiers in Immunology·Gestur VidarssonTheo Rispens
Nov 18, 2014·Frontiers in Immunology·Clark L Anderson
Sep 16, 2014·Frontiers in Immunology·Kristi BakerRichard S Blumberg
Oct 27, 2015·Immunological Reviews·Nigel M StapletonGestur Vidarsson
Sep 21, 2016·Histochemistry and Cell Biology·Rebecca MöllerE Michael Danielsen
Jan 11, 2014·Journal of the American Society of Nephrology : JASN·Landon E DicksonBruce A Molitoris
Nov 15, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maximilian BrinkhausGestur Vidarsson
Jul 3, 2021·International Journal of Molecular Sciences·Maxime FieuxEmilie Bequignon

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Methods Mentioned

BETA
electron microscopy
electron tomography
gel filtration

Software Mentioned

IMOD
SerialEM

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