Electron transfer and electrocatalytic properties of the immobilized methionine80alanine cytochrome c variant

The Journal of Physical Chemistry. B
Stefano CasaliniMarco Sola

Abstract

The M80A variant of yeast iso-1-cytochrome c (cytc), which features a noncoordinating Ala residue in place of the axial heme iron Met ligand, was chemisorbed on a gold electrode coated with 4-mercaptopyridine or carboxyalkanethiol self-assembled monolayers (SAM) and investigated by cyclic voltammetry at varying conditions of temperature, pH, and O2 concentration. The E degrees ' value (standard reduction potential for the heme Fe(III)/Fe(II) couple) of M80A cytc on both SAMs is of approximately -200 mV (vs the standard hydrogen electrode, SHE) at pH 7, which is more than 400 mV lower than that of native cytochrome c in the same conditions. The thermodynamics of Fe(III) to Fe(II) reduction and the kinetics of heterogeneous electron transfer (ET) are dominated by the presence of a hydroxide ion as the sixth axial heme iron ligand above pH 6. On both SAMs, protonation of the bound hydroxide ion is mainly responsible for the changes in these parameters at low pH, since the distances of ET between the heme and the electrode are found to be independent of pH in the range of 5-11. The invariance of the electrochemical features up to pH 11 indicates that no changes in heme iron coordination occur at high pH, at variance with native cyt...Continue Reading

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Citations

Mar 23, 2012·Antioxidants & Redox Signaling·Shradha PrabhulkarChen-Zhong Li
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
Apr 4, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Giulia Di RoccoMarco Sola
Oct 22, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Patrícia M Paes de SousaMargarida M Correia dos Santos
Jan 30, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Antonio RanieriMarco Sola
Oct 11, 2016·Chemical Society Reviews·Stefano CasaliniFabio Biscarini
Jan 28, 2016·Journal of Inorganic Biochemistry·Jonathan HusbandJeffrey J Warren
Feb 12, 2014·Metallomics : Integrated Biometal Science·Antonio RanieriMarco Sola
Jul 5, 2013·Physical Chemistry Chemical Physics : PCCP·Giulia Di RoccoMarco Sola
Jan 19, 2012·Physical Chemistry Chemical Physics : PCCP·Luca MeddaEdmond Magner
Oct 8, 2020·Data in Brief·Marcello PignataroFederica Del Monte
Oct 16, 2008·Journal of the American Chemical Society·Stefano CasaliniMarco Sola
Jan 12, 2010·The Journal of Physical Chemistry. B·Stefano CasaliniMarco Sola
Oct 28, 2017·Journal of Inorganic Biochemistry·Marzia BelleiGianantonio Battistuzzi
Jul 11, 2012·Biochemistry·Gianantonio BattistuzziMarco Sola
Oct 14, 2017·Chemical Reviews·Damián Alvarez-PaggiDaniel H Murgida
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio RanieriGianantonio Battistuzzi

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