Electron transfer between the QmoABC membrane complex and adenosine 5'-phosphosulfate reductase

Biochimica Et Biophysica Acta
Américo G DuarteInês A C Pereira

Abstract

The dissimilatory adenosine 5'-phosphosulfate reductase (AprAB) is a key enzyme in the sulfate reduction pathway that catalyzes the reversible two electron reduction of adenosine 5'-phosphosulfate (APS) to sulfite and adenosine monophosphate (AMP). The physiological electron donor for AprAB is proposed to be the QmoABC membrane complex, coupling the quinone-pool to sulfate reduction. However, direct electron transfer between these two proteins has never been observed. In this work we demonstrate for the first time direct electron transfer between the Desulfovibrio desulfuricans ATCC 27774 QmoABC complex and AprAB. Cyclic voltammetry conducted with the modified Qmo electrode and AprAB in the electrolyte solution presented the Qmo electrochemical signature with two additional well-defined one electron redox processes, attributed to the AprAB FAD redox behavior. Moreover, experiments performed under catalytic conditions using the QmoABC modified electrode, with AprAB and APS in solution, show a catalytic current peak develop in the cathodic wave, attributed to substrate reduction, and which is not observed in the absence of QmoABC. Substrate dependence conducted with different electrode preparations (with and without immobilized Q...Continue Reading

References

Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Günter FritzUlrich Ermler
Aug 9, 2003·Biochimica Et Biophysica Acta·Ricardo H PiresInês A C Pereira
May 8, 2008·Nature Reviews. Microbiology·Gerard Muyzer, Alfons J M Stams
Nov 11, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hideaki OgataWolfgang Lubitz
Jul 13, 2011·Frontiers in Microbiology·Inês A Cardoso PereiraSofia Santos Venceslau
Jun 27, 2013·Journal of the American Chemical Society·Duncan G G McMillanLars J C Jeuken

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Citations

Aug 6, 2020·MSystems·Christopher B TrivediJohn R Spear
Jan 6, 2021·Chemical Reviews·Filipa CalistoManuela M Pereira
Jan 19, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Lena AppelMatthias Boll
Mar 24, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Américo G DuarteInês A C Pereira

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