PMID: 3753651Jan 28, 1986Paper

Electron-transfer complexes of Ascaris suum muscle mitochondria. II. Succinate-coenzyme Q reductase (complex II) associated with substrate-reducible cytochrome b-558

Biochimica Et Biophysica Acta
Shinzaburo TakamiyaH Oya

Abstract

A succinate-coenzyme Q reductase (complex II) was isolated in highly purified form from Ascaris muscle mitochondria by detergent solubilization, ammonium sulfate fractionation and gel filtration on a Sephadex G-200 column. The enzyme preparation catalyzes electron transfer from succinate to coenzyme Q1 with a specific activity of 1.2 mumol coenzyme Q1 reduced per min per mg protein at 25 degrees C. The isolated complex II is essentially free of NADH-ferricyanide reductase, reduced CoQ2-cytochrome c reductase and cytochrome c oxidase and consists of four major polypeptides with apparent molecular weights of 66 000, 27 000, 12 000 and 11 000 and two minor ones with Mr of 36 000 and 16 000. The complex II contained cytochrome b-558, a major constituent cytochrome of Ascaris mitochondria, at a concentration of 3.6 nmol per mg protein, but neither other cytochromes nor quinone. The cytochrome b-558 in the complex II was reduced with succinate. In the presence of Ascaris NADH-cytochrome c reductase (complex I-III) (Takamiya, S., Furushima, R. and Oya, H. (1984) Mol. Biochem. Parasitol. 13, 121-134), the cytochrome b-558 in complex II was also reduced with NADH and reoxidized with fumarate. These results suggest the cytochrome b-558 t...Continue Reading

References

Oct 23, 1978·Biochimica Et Biophysica Acta·A Kröger
Apr 14, 1975·Biochimica Et Biophysica Acta·K S Cheah
Aug 13, 1968·Biochemical and Biophysical Research Communications·I Y Lee, B Chance
Nov 3, 1970·Biochimica Et Biophysica Acta·K S Cheah, B Chance
Aug 4, 1970·Biochimica Et Biophysica Acta·M A Wilson, J Cascarano
Apr 27, 1971·Biochimica Et Biophysica Acta·G C HillN W Mathewson
Nov 30, 1982·Biochimica Et Biophysica Acta·R A Capaldi
Jan 1, 1981·Annual Review of Physiology·H J Saz
Apr 1, 1980·Molecular and Biochemical Parasitology·P Köhler, R Bachmann
Dec 1, 1959·Biochimica Et Biophysica Acta·G KIKUCHIE S GUZMAN BARRON
Aug 5, 1961·Biochimica Et Biophysica Acta·G KIKUCHI, S BAN

❮ Previous
Next ❯

Citations

Apr 15, 1986·Experientia·P Köhler
Mar 1, 1988·Molecular and Biochemical Parasitology·C F Fioravanti, Y Kim
Feb 1, 1989·Molecular and Biochemical Parasitology·T CampbellR Komuniecki
Jul 1, 1996·Molecular and Biochemical Parasitology·S TakamiyaT Aoki
Jun 1, 1997·International Journal for Parasitology·K KitaS Takamiya
May 18, 2001·Molecular and Biochemical Parasitology·I Patricia del ArenalJ Edgardo Escamilla
Feb 8, 1993·Biochimica Et Biophysica Acta·S TakamiyaT Aoki
Jan 15, 1990·Biochemical and Biophysical Research Communications·K KitaM Kasahara
Dec 1, 1991·Bioscience Reports·V P Skulachev
Jan 23, 2002·Biochimica Et Biophysica Acta·Bernard D Lemire, Kayode S Oyedotun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ascariasis

Ascariasis is a helminthic infection of global distribution with more than 1.4 billion persons infected throughout the world. Here is the latest research.

Ascariasis (ASM)

Ascariasis is a helminthic infection of global distribution with more than 1.4 billion persons infected throughout the world. Here is the latest research.