Electron Transfer Dissociation and Collision-Induced Dissociation of Underivatized Metallated Oligosaccharides

Journal of the American Society for Mass Spectrometry
Ranelle M Schaller-DukeCarolyn J Cassady

Abstract

Electron transfer dissociation (ETD) and collision-induced dissociation (CID) were used to investigate underivatized, metal-cationized oligosaccharides formed via electrospray ionization (ESI). Reducing and non-reducing sugars were studied including the tetrasaccharides maltotetraose, 3α,4β,3α-galactotetraose, stachyose, nystose, and a heptasaccharide, maltoheptaose. Univalent alkali, divalent alkaline earth, divalent and trivalent transition metal ions, and a boron group trivalent metal ion were adducted to the non-permethylated oligosaccharides. ESI generated [M + Met]+, [M + 2Met]2+, [M + Met]2+, [M + Met - H]+, and [M + Met - 2H]+ most intensely along with low intensity nitrate adducts, depending on the metal and sugar ionized. The ability of these metal ions to produce oligosaccharide adduct ions by ESI had the general trend: Ca(II) > Mg(II) > Ni(II) > Co(II) > Zn(II) > Cu(II) > Na(I) > K(I) > Al(III) ≈ Fe(III) ≈ Cr(III). Although trivalent metals were utilized, no triply charged ions were formed. Metal cations allowed for high ESI signal intensity without permethylation. ETD and CID on [M + Met]2+ produced various glycosidic and cross-ring cleavages, with ETD producing more cross-ring and internal ions, which are useful f...Continue Reading

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Citations

Jan 2, 2021·Computational and Structural Biotechnology Journal·Sandrien DesmetKris Morreel
May 29, 2018·Journal of the American Society for Mass Spectrometry·Kelsey A MorrisonBrian H Clowers
Jun 29, 2021·Journal of Mass Spectrometry : JMS·Zachary J SasieneGlen P Jackson
Sep 17, 2020·Analytical Chemistry·Rayan MurtadaJinshan Gao
Apr 21, 2020·Journal of the American Society for Mass Spectrometry·Hagen Buck-WieseDavid Ropartz

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