Electron transfer in the substrate-dependent suicide inactivation of lysine 5,6-aminomutase

Biochemistry
K H TangPerry A Frey

Abstract

The lysine 5,6-aminomutase (5,6-LAM) purified from Clostridium sticklandii was found to undergo rapid inactivation in the absence of the activating enzyme E(2) and ATP. In the presence of substrate, inactivation was also seen for the recombinant 5,6-LAM. This adenosylcobalamin-dependent enzyme is postulated to generate cob(II)alamin and the 5'-deoxyadenosyl radical through enzyme-induced homolytic scission of the Co-C bond. However, the products cob(III)alamin and 5'-deoxyadenosine were observed upon inactivation of 5,6-LAM. Cob(III)alamin production, as monitored by the increase in A(358), proceeds at the same rate as the loss of enzyme activity, suggesting that the activity loss is related to the adventitious generation of cob(III)alamin during enzymatic turnover. The cleavage of adenosylcobalamin to cob(III)alamin is accompanied by the formation of 5'-deoxyadenosine at the same rate, and the generation of cob(III)alamin proceeds at the same rate both aerobically and anaerobically. Suicide inactivation requires the presence of substrate, adenosylcobalamin, and PLP. We have ruled out the involvement of either the putative 5'-deoxyadenosyl radical or dioxygen in suicide inactivation. We have shown that one or more reaction inte...Continue Reading

References

Jan 28, 1999·The Journal of Biological Chemistry·T Toraya, K Mori

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Citations

Feb 26, 2013·Photosynthesis Research·Joseph Kuo-Hsiang TangSun W Tam
Dec 17, 2003·Current Opinion in Structural Biology·George H Reed, Steven O Mansoorabadi
Oct 25, 2008·The Journal of Biological Chemistry·Kirsten R WolthersNigel S Scrutton
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Feb 25, 2014·International Journal of Molecular Sciences·Amarendra Nath MaityShyue-Chu Ke
Oct 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Frederick BerkovitchCatherine L Drennan
May 15, 2012·Annual Review of Biophysics·Daniel P DowlingCatherine L Drennan
Apr 12, 2011·Trends in Biotechnology·Bian WuDick B Janssen
Mar 26, 2011·Biochimica Et Biophysica Acta·Perry A Frey, George H Reed
Jan 16, 2007·Biochimica Et Biophysica Acta·Frank J Ruzicka, Perry A Frey
Dec 15, 2011·The Journal of Biological Chemistry·Valentin Cracan, Ruma Banerjee
Sep 9, 2011·Bioorganic Chemistry·Caitlyn MakinsKirsten R Wolthers
Sep 15, 2009·Chemical Reviews·Israel FernándezMiguel A Sierra
Aug 19, 2009·The Journal of Physical Chemistry. B·Amarendra N MaityShyue-Chu Ke
Aug 10, 2006·Chemical Reviews·Perry A FreyGeorge H Reed
Jun 9, 2005·Chemical Reviews·Kenneth L Brown
Sep 19, 2003·Archives of Biochemistry and Biophysics·Kuo-Hsiang TangPerry A Frey
Jul 9, 2010·The Journal of Physical Chemistry. B·Frank J Ruzicka, Perry A Frey

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