Electronegative L5-LDL induces the production of G-CSF and GM-CSF in human macrophages through LOX-1 involving NF-κB and ERK2 activation

Atherosclerosis
Tzu-Ching YangShao-Chun Lu

Abstract

Circulating levels of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF) are associated with the severity of acute myocardial infarction (AMI). However, what causes increases in G-CSF and GM-CSF is unclear. In this study, we investigated whether L5-low-density lipoprotein (LDL), a mildly oxidized LDL from AMI, can induce G-CSF and GM-CSF production in human macrophages. L1-LDL and L5-LDL were isolated through anion-exchange chromatography from AMI plasma. Human macrophages derived from THP-1 and peripheral blood mononuclear cells were treated with L1-LDL, L5-LDL, or copper-oxidized LDL (Cu-oxLDL) and G-CSF and GM-CSF protein levels in the medium were determined. In addition, the effects of L5-LDL on G-CSF and GM-CSF production were tested in lectin-type oxidized LDL receptor-1 (LOX-1), CD36, extracellular signal-regulated kinase (ERK) 1, and ERK2 knockdown THP-1 macrophages. L5-LDL but not L1-LDL or Cu-oxLDL significantly induced production of G-CSF and GM-CSF in macrophages. In vitro oxidation of L1-LDL and L5-LDL altered their ability to induce G-CSF and GM-CSF, suggesting that the degree of oxidation is critical for the effects. Knockdown and antibody neutralization ex...Continue Reading

Citations

Mar 2, 2019·The Journal of Experimental Medicine·Torben MentrupBernd Schröder
Jul 6, 2019·Frontiers in Immunology·Noushin LotfiNafiseh Esmaeil
Sep 20, 2019·Mediators of Inflammation·Po-Yuan ChangShao-Chun Lu
Jun 20, 2018·International Journal of Molecular Sciences·Virginia Actis Dato, Gustavo Alberto Chiabrando
Jul 25, 2019·Clinical Science·B Sumayyah H Sokeechand, Bernardo L Trigatti
Dec 4, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuan WangXian K Zhang
Apr 1, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lirong YiJianxiang Liu
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yao QingLiu Yanhui

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