PMID: 2983672Jan 15, 1985

Electroneutral efflux of Ca2+ from liver mitochondria

The Biochemical Journal
M D Brand

Abstract

Respiring liver mitochondria were allowed to export Ca2+ on the endogenous Ca2+/nH+ antiporter in the presence of Ruthenium Red (to inhibit uptake on the Ca2+ uniporter) until a steady state was reached. Addition of sufficient of the ionophore A23187 (which catalyses Ca2+/2H+ exchange) to bring the Ca2+ and H+ gradients into equilibrium did not alter the steady state. Thermodynamic analysis showed that if a Ca2+/nH+ exchange with any value of n other than 2 was at equilibrium, addition of A23187 would have caused an easily measurable change in extramitochondrial free [Ca2+]. Therefore, the endogenous carrier of liver mitochondria catalyses electroneutral Ca2+/2H+ antiport.

Citations

Mar 21, 2012·Cell Calcium·Raz Palty, Israel Sekler
Nov 1, 1988·Biological Reviews of the Cambridge Philosophical Society·J G Altin, F L Bygrave
May 1, 1987·Biological Reviews of the Cambridge Philosophical Society·M D Brand, M P Murphy
Jun 2, 1986·European Journal of Biochemistry·A J MoodyP Mitchell
Jan 24, 2009·Biochimica Et Biophysica Acta·T E Gunter, Shey-Shing Sheu
May 15, 1988·Archives of Biochemistry and Biophysics·D W JungG P Brierley
Nov 23, 1989·Biochimica Et Biophysica Acta·M P Murphy
Jan 13, 1995·The Journal of Biological Chemistry·D W JungG P Brierley
Oct 1, 1994·Journal of Bioenergetics and Biomembranes·K K Gunter, T E Gunter
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Jan 17, 2020·International Journal of Molecular Sciences·Chinatsu NakagawaYoshihiro Suzuki-Karasaki
Oct 13, 2020·Frontiers in Physiology·Gayathri K NatarajanWai-Meng Kwok

Related Concepts

Calcimycin
Calcium
Resting Potentials
Mitochondria, Liver
Protons
Thermodynamics

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