Electroneutral Na-coupled cotransporter expression in the kidney during variations of NaCl and water metabolism

Hypertension
G MorenoGerardo Gamba

Abstract

The purpose of the present study was to analyze the long-term regulation of renal bumetanide-sensitive Na+-K+-2Cl- cotransporter and thiazide-sensitive Na+-Cl- cotransporter gene expression during changes in NaCl and water metabolism. Male Wistar rats exposed to high or low NaCl intake, saline loading, dehydration, water loading, and furosemide administration during 7 days were studied. Control groups had access to regular food and tap water. Rats were kept in metabolic cages for 4 days before and during the experiment to determine daily urinary electrolyte excretion and osmolarity. At the end of the experiment, creatinine clearance and serum electrolyte levels were also measured. Kidneys were excised and macroscopically subdivided into cortex and outer and inner medulla. Total RNA was extracted from each individual cortex or outer medulla by use of the guanidine/cesium chloride method. The Na+-K+-2Cl- cotransporter expression in outer medulla total RNA was assessed by nonradioactive Northern blot analysis and the Na+-Cl- cotransporter expression in renal cortex total RNA was assessed by semiquantitative polymerase chain reaction. Experimental maneuvers were adequately tolerated, and all groups developed the appropriate renal r...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·F A Gesek, P A Friedman
Jul 1, 1990·Journal of the American Society of Nephrology : JASN·Z F ChenD D Fanestil
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A Payne, B Forbush
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C XuB Forbush
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G GambaS C Hebert
Jul 1, 1996·Kidney International·M D PlotkinS C Hebert
Jan 1, 1996·Annual Review of Physiology·M R KaplanE Delpire

❮ Previous
Next ❯

Citations

Oct 3, 1999·Kidney International·G Gamba
Feb 9, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Katy Sánchez-PozosNorma A Bobadilla
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·M A Knepper, H L Brooks
Jul 24, 2012·Current Opinion in Nephrology and Hypertension·María Castañeda-Bueno, Gerardo Gamba
Mar 1, 2008·Clinical and Experimental Pharmacology & Physiology·Caroline RugaleBernard Jover
Oct 5, 2012·American Journal of Physiology. Renal Physiology·Gerardo Gamba
Nov 27, 2004·American Journal of Nephrology·Gheun-Ho Kim
Aug 20, 2011·Journal of the American Society of Nephrology : JASN·Juan Pablo ArroyoOlivier Staub
May 16, 2001·Biochemical and Biophysical Research Communications·S MacKenzieW Lockette
Feb 7, 2007·Journal of Voice : Official Journal of the Voice Foundation·Mahalakshmi Sivasankar, Kimberly V Fisher
Oct 9, 2004·Biochimica Et Biophysica Acta·Horacio J NovairaMarcelo M Morales
Aug 26, 2004·American Journal of Physiology. Renal Physiology·Joyce TrujilloNorma A Bobadilla
Jan 3, 2014·Physiology·Caroline Ronzaud, Olivier Staub
Apr 14, 2011·Physiology·Juan Pablo ArroyoGerardo Gamba
Oct 4, 2013·American Journal of Physiology. Renal Physiology·Zesergio MeloGerardo Gamba
Jan 5, 2000·Physiological Reviews·R F Reilly, D H Ellison
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Jakob NielsenMark A Knepper
Mar 10, 1999·The American Journal of Physiology·D D FanestilP Blakely
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Johannes Loffing, Brigitte Kaissling
Mar 4, 2003·Journal of Applied Physiology·Lian WangDonovan B Yeates
Jun 26, 2001·Journal of the American Society of Nephrology : JASN·Joseph G AbdallahDavid H Ellison

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.