Electronic cigarette vapor increases Streptococcus mutans growth, adhesion, biofilm formation, and expression of the biofilm-associated genes.

Oral Diseases
Mahmoud Rouabhia, Abdelhabib Semlali

Abstract

It still not known whether electronic cigarettes (e-cigarettes) contribute to dental caries. This study aimed to evaluate the effect of e-cigarettes on the growth of Streptococcus mutans, the formation of biofilm, and the expression of certain virulence genes. Streptococcus mutans cells were exposed or not to e-cigarettes with and without nicotine or to cigarette smoke twice a day for 15 min each exposure period. The bacterial growth and the expression of glucosyltranferase, competence, and glucan-binding genes were evaluated after 24 hr. Biofilm formation was assessed after 1, 2, and 3 days. S. mutans adhesion and growth to e-cigarette exposed human teeth were assessed. We observed an increase in S. mutans growth with e-cigarettes, mainly at the early culture period. This was confirmed by an increase of biofilm mass ranging from 8 ± 0.5 mg with the control to 47 ± 5 mg after six exposures to nicotine-rich e-cigarettes. S. mutans cells adhered better to e-cigarette exposed teeth. E-cigarettes increased the expression of glucosyltranferase, competence, and glucan-binding genes. E-cigarettes increased the growth of S. mutans and the expression of virulent genes. E-cigarettes promoted the adhesion to, and formation of biofilms on ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 12, 2004·Journal of Cancer Research and Clinical Oncology·H FujikiM Suganuma
Oct 30, 2007·Journal of Medical Microbiology·Moshe ShemeshDoron Steinberg
Jan 12, 2008·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Ali Taghavi ZonuzRamin Mostofi Zadeh Farahani
Oct 5, 2010·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Fernanda Brasil BaboniEdvaldo Antonio Ribeiro Rosa
Jul 21, 2012·European Journal of Oral Sciences·Ruijie HuangRichard L Gregory
Dec 25, 2012·Chemistry & Biology·Hwang-Soo Joo, Michael Otto
May 2, 2013·Archives of Oral Biology·Rodrigo A GiacamanRamiro J Castro
Oct 25, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W KrzyściakA Skalniak
Sep 14, 2014·Applied and Environmental Microbiology·R HuangR L Gregory
Jan 31, 2016·Applied and Environmental Microbiology·Xuelian HuangRobert A Burne
Jun 28, 2016·Biochemical and Biophysical Research Communications·Chad A LernerIrfan Rahman
Aug 31, 2018·The Journal of Oral Implantology·Mahmoud RouabhiaReginaldo Bruno Gonçalves
Sep 8, 2018·PloS One·Shin Ae KimJeffrey J Kim
Jan 24, 2019·International Journal of Environmental Research and Public Health·Humidah AlanaziMahmoud Rouabhia
Feb 6, 2019·Journal of Medical Internet Research·Kahlia McCauslandJonine Jancey
Apr 12, 2019·International Journal of Environmental Research and Public Health·Nkiruka C AtuegwuEric M Mortensen
May 17, 2019·International Journal of Environmental Research and Public Health·Giancarlo A CuadraDominic L Palazzolo
Sep 26, 2019·Journal of Oral Microbiology·Nasreen F El-Ezmerli, Richard L Gregory

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Citations

Apr 3, 2021·BMJ Case Reports·Michelle Massey, Joseph Barney

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