Electronic Pneumatic Injection-Assisted Dermal Drug Delivery Visualized by Ex Vivo Confocal Microscopy.

Lasers in Surgery and Medicine
Liora BikUffe H Olesen

Abstract

Electronic pneumatic injection (EPI) is a technique for dermal drug delivery, which is increasingly being used in clinical practice. However, only few studies have been reported on cutaneous drug distribution and related clinical endpoints. We aimed to visualize the immediate cutaneous drug distribution, changes in skin architecture, and related clinical endpoint of EPI. Acridine orange (AO) solution was administered to ex vivo porcine skin by EPI at pressure levels from 4 to 6 bar with a fixed injection volume of 50 µl and nozzle size of 200 µm. Immediate cutaneous distribution was visualized using ex vivo confocal microscopy (EVCM). Changes in skin architecture were visualized using both EVCM and hematoxylin and eosin-stained cryosections. The defined immediate endpoint was a clinically visible papule formation on the skin. The pressure threshold to consistently induce a papule was 4 bar, achieving delivery of AO to the deep dermis (2319 µm axial and 5944 µm lateral distribution). Increasing the pressure level to 6 bar did not lead to significant differences in axial and lateral dispersion (P = 0.842, P = 0.905; respectively). A distinctively hemispherical distribution pattern was identified. Disruption of skin architecture o...Continue Reading

References

Dec 3, 2002·Pharmaceutical Research·Joy Schramm, Samir Mitragotri
Jun 24, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joy Schramm-Baxter, Samir Mitragotri
Jul 7, 2007·Journal of Biomedical Optics·Yogesh G PatelMilind Rajadhyaksha
Nov 22, 2008·Journal of Biomedical Optics·Daniel S GareauMilind Rajadhyaksha
Jun 25, 2010·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jin Woong LeeChang Kyun Lee
Oct 5, 2010·Aesthetic Surgery Journal·Kazimierz F Kobus, Tomasz Dydymski
Nov 26, 2010·International Journal of Dermatology·Alex LevenbergMoshe Lapidoth
Apr 5, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yasunari Michinaka, Samir Mitragotri
Jun 4, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Tae Young HanChang Kyun Lee
Nov 23, 2012·International Journal of Dermatology·Hyun Kyu KimSung Eun Kim
Oct 15, 2013·The British Journal of Dermatology·A BennàssarJ Malvehy
Jan 1, 2014·Journal of Cutaneous Medicine and Surgery·Melissa Nantel-BattistaAntranik Benohanian
Feb 24, 2015·Journal of Biomedical Optics·Eileen S FloresMilind Rajadhyaksha
May 26, 2015·Expert Opinion on Drug Delivery·Nora C HoganIan W Hunter
Jun 2, 2015·International Journal of Pharmaceutics·Aurélie SchoubbenLucio Postrioti
Jun 23, 2015·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·A K DąbrowskaR M Rossi
Sep 26, 2015·Journal of Drug Targeting·Kevin Ita
Apr 18, 2016·Journal of the American Academy of Dermatology·Molly WannerR Rox Anderson
Jul 20, 2016·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·S B ChoB J Kim
Oct 4, 2016·Dermatologic Clinics·Caterina LongoJosep Malvehy Guilera
Nov 29, 2016·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Sophie VadeboncoeurAntranik Benohanian
May 12, 2018·Clinical, Cosmetic and Investigational Dermatology·Daniel Barolet, Antranik Benohanian
May 23, 2018·Dermatology Practical & Conceptual·Elisa CinottiPietro Rubegni
Aug 31, 2018·Journal of Oral and Maxillofacial Pathology : JOMFP·Syed Ahmed TaqiSyed Ahmed Zaki
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tejashree WaghuleKamal Dua
Jun 30, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jonathan A SimmonsJeremy O Marston
Jul 16, 2019·International Journal of Pharmaceutics·Pankaj Rohilla, Jeremy O Marston
Oct 16, 2019·Frontiers in Oncology·Vadim A ByvaltsevAlexander A Potapov
Dec 4, 2019·Lasers in Surgery and Medicine·Andrés M ErlendssonAnthony M Rossi

❮ Previous
Next ❯

Citations

Aug 25, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Suliman KhanTimo L M Ten Hagen
Sep 5, 2021·Drug Delivery and Translational Research·Abdul Mohizin, Jung Kyung Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
confocal microscopy
biopsies
biopsy
imaging technique

Software Mentioned

EVCM
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
A LeclercqFrédéric Cambazard
Dermatology Practical & Conceptual
Elisa CinottiPietro Rubegni
Archives of Dermatology
D J Schissel, C Sartori
© 2022 Meta ULC. All rights reserved