Electronic spectroscopy of cold, protonated tryptophan and tyrosine

Journal of the American Chemical Society
Oleg V BoyarkinThomas R Rizzo

Abstract

We report here a new method to obtain electronic spectra of biomolecular ions that are produced in the gas phase by electrospray and cooled to approximately 10 K in a 22-pole ion trap, and we demonstrate this technique by applying it to protonated tryptophan and tyrosine. Cooling in the trap greatly simplifies the spectrum of protonated tyrosine, which exhibits a well-defined band origin and clearly resolved low frequency vibrational bands. In contrast, the spectrum of protonated tryptophan exhibits only broad features, even at low temperatures, suggesting that a fast nonradiative process broadens the individual vibronic features, even upon excitation at the electronic band origin. The method demonstrated here should be applicable to a wide variety of biological molecules.

References

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Citations

Mar 31, 2012·Journal of the American Society for Mass Spectrometry·Tobias N WassermannThomas R Rizzo
Apr 25, 2012·Journal of the American Society for Mass Spectrometry·Georgios PapadopoulosThomas R Rizzo
Aug 27, 2013·Accounts of Chemical Research·Arron B WolkMark A Johnson
Feb 16, 2010·Faraday Discussions·D Gerlich, G Borodi
Jan 1, 2011·Faraday Discussions·Georgios PapadopoulosThomas R Rizzo
Aug 7, 2010·The Review of Scientific Instruments·Annette SvendsenThomas R Rizzo
Dec 25, 2007·The Journal of Chemical Physics·Akira TerasakiTamotsu Kondow
Aug 7, 2010·The Review of Scientific Instruments·Oskar AsvanyStephan Schlemmer
Dec 3, 2008·The Journal of Chemical Physics·Umesh KadhaneHenning Zettergren
Oct 24, 2007·The Journal of Chemical Physics·Jaime A StearnsThomas R Rizzo
Jan 3, 2013·The Journal of Chemical Physics·Christian HockDaniel M Neumark
May 2, 2008·The Journal of Chemical Physics·B LucasS Brondsted Nielsen
Oct 9, 2007·The Journal of Chemical Physics·V LepèreA Fujii
Apr 3, 2014·The Review of Scientific Instruments·Oleg V Boyarkin, Vladimir Kopysov
Nov 18, 2009·The Journal of Chemical Physics·Dawei ZhaoOtto Dopfer
Jan 10, 2016·The Journal of Chemical Physics·Caroline SeaibyThomas R Rizzo
Feb 26, 2009·Mass Spectrometry Reviews·Nick C Polfer, Jos Oomens
Mar 16, 2016·Journal of the American Society for Mass Spectrometry·Adam P CismesiaNicolas C Polfer
Nov 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Soojin ParkHan Bin Oh
Mar 15, 2016·Journal of the American Chemical Society·Luke MacAleesePhilippe Dugourd
Oct 23, 2015·The Journal of Physical Chemistry. a·Yoshiya InokuchiThomas R Rizzo
Aug 25, 2015·The Journal of Chemical Physics·Natalia Esteves-LópezChristophe Jouvet
Oct 10, 2014·The Journal of Chemical Physics·Claude DedonderChristophe Jouvet
Jul 18, 2015·The Journal of Physical Chemistry. a·Yoshiya InokuchiTakayuki Ebata
Jun 21, 2015·Journal of the American Society for Mass Spectrometry·Antoine MassonThomas R Rizzo
Jul 3, 2015·The Journal of Physical Chemistry. a·Yoshiya InokuchiThomas R Rizzo
Jun 29, 2016·The Journal of Physical Chemistry. a·Aleksandr Y PereverzevOleg V Boyarkin
Aug 20, 2016·The Journal of Physical Chemistry. a·Andrew F DeBlaseTimothy S Zwier
Sep 15, 2016·The Journal of Physical Chemistry. B·Hiroki OtakiYuji Sugita
Dec 21, 2016·Inorganic Chemistry·Yoshiya InokuchiThomas R Rizzo
Jul 17, 2016·The Journal of Chemical Physics·Shuang XuJ Mathias Weber
Apr 8, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Reza OmidyanGholamhassan Azimi
Apr 6, 2017·Chemistry, an Asian Journal·Jennifer Anna NobleChristophe Jouvet
May 5, 2017·Physical Chemistry Chemical Physics : PCCP·Yoshiya InokuchiTakayuki Ebata
May 26, 2017·The Journal of Physical Chemistry. a·Yogeshwaran KrishnanManikandan Paranjothy
Mar 10, 2017·Physical Chemistry Chemical Physics : PCCP·R Lozada GarciaG Grégoire

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