Electronic transduction of HIV-1 drug resistance in AIDS patients

Chembiochem : a European Journal of Chemical Biology
Lital AlfontaI Willner

Abstract

A drug composition consisting of nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), and protease inhibitors (PIs) is commonly used in AIDS therapy. A major difficulty encountered with the therapeutic composite involves the emergence of drug-resistant viruses, especially to the PIs, regarded as the most effective drugs in the composition. We present a novel bioelectronic means to detect the appearance of mutated HIV-1 exhibiting drug resistance to the PI saquinavir. The method is based on the translation of viral RNA, the association of cleaved or uncleaved Gag polyproteins at an electrode surface functionalized with the respective antibodies, and the bioelectronic detection of the Gag polyproteins associated with the surface. The bioelectronic process includes the association of anti-MA or anti-CA antibodies, the secondary binding of an antibody-horseradish peroxidase (HRP) conjugate, and the biocatalyzed precipitation of an insoluble product on the electronic transducers. Faradaic impedance measurements and quartz crystal microbalance analyses are employed to follow the autoprocessing of the Gag polyproteins. The method was applied to determine drug resistance in infe...Continue Reading

References

Apr 20, 1990·Science·N A RobertsA Kröhn
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N E KohlI S Sigal
Jan 8, 1997·JAMA : the Journal of the American Medical Association·S G DeeksJ O Kahn
Apr 25, 1997·Biochimica Et Biophysica Acta·S I WilsonJ Kay
Nov 26, 1997·Antimicrobial Agents and Chemotherapy·U NillrothU H Danielson
Nov 26, 1999·Nucleic Acids Research·S O KelleyM G Hill
Oct 4, 2000·Nature Biotechnology·E M BoonJ K Barton
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·K HabermüllerW Schuhmann
May 18, 2001·Trends in Biotechnology·I Willner, B Willner
May 30, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·N KochN Yahi
Jun 9, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J G García-Lerma, W Heneine
Jan 31, 2002·Journal of the American Chemical Society·Fernando PatolskyItamar Willner
Apr 12, 2002·Biochemical and Biophysical Research Communications·Immanuel BlumenzweigMoshe Kotler

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