Electronmicroscopy and circular dichroism of the dynamics of the formation and dissolution of supramolecular forms of Z-DNA

Journal of Biomolecular Structure & Dynamics
H CastlemanB F Erlanger

Abstract

Previous electronmicroscopic studies had shown that N-acetylaminofluorene (AFF)-substituted poly(dG-dC)-poly(dG-dC) in the Z conformation, in 10mM Mg++, condensed into periodically banded, branched structures. We now show that similar structures are seen when poly(dG-dC)-poly(dG-dC) is converted to the Z conformation by heating to 60 degrees C in 1mM Mn++ or to 65 degrees in the presence of 0.5mM Mn++. We demonstrate that these banded structures form in solution, i.e. they are not artifacts of the preparative procedures used for electronmicroscopy, by crosslinking the Z conformers in solution with DL-diepoxybutane (DEB), and then restoring the solution to conditions that favor return to the B conformation. Circular dichroism (CD) and immunochemical studies showed that the Z conformation was maintained and the banded supramolecular structures were still seen by electronmicroscopy. Electronmicroscopy and CID were also used to follow the dissolution of the supramolecular structures by controlled scission of the crosslinks with the eventual return to the short double stranded molecules typical of the B conformers. During this process, supercoiled structures, both toroidal and interwound, were observed. The relationship of the toroi...Continue Reading

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C J Benham
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·R D ColeM W Hsiang
May 25, 1978·Journal of Molecular Biology·D K ChattorajA Schellman
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·U K Laemmli
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H J VollenweiderJ M Sogo
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J D Griffith
Jan 1, 1974·Journal of Supramolecular Structure·H P Erickson
Jun 20, 1972·Journal of Molecular Biology·R F Whiting, F P Ottensmeyer
Apr 14, 1967·Journal of Molecular Biology·P D Lawley, P Brookes
Oct 27, 1970·Biochemistry·M HaynesW B Gratzer
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K A Marx, T C Reynolds
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·T M JovinM Robert-Nicoud
Jan 1, 1983·CRC Critical Reviews in Biochemistry·P J Butler
Jul 1, 1980·Archives of Microbiology·V Virrankoski-Castrodeza, J H Parish
Dec 25, 1980·Journal of Molecular Biology·J Widom, R L Baldwin
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G W BradyC J Benham
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Nordheim, A Rich
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L J Peck, J C Wang
Nov 1, 1983·Biopolymers·C J Benham
Dec 10, 1983·Nucleic Acids Research·H CastlemanB F Erlanger
Mar 13, 1984·Biochemistry·J H ShibataV Knauf
Mar 25, 1983·Nucleic Acids Research·T J Thomas, V A Bloomfield
Oct 25, 1982·Journal of Molecular Biology·C H Lee, E Charney
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J A PETRUSKA, A J HODGE

❮ Previous
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Citations

Dec 22, 1986·Nucleic Acids Research·V P AntaoR L Ratliff
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Abraham Minsky
Jan 1, 1990·Biopolymers·G E PlumV A Bloomfield
Aug 1, 1989·Journal of Biomolecular Structure & Dynamics·G S Manning
Jun 1, 1988·Journal of Biomolecular Structure & Dynamics·J B Chaires, M T Norcum
Feb 1, 1992·Journal of Biomolecular Structure & Dynamics·E Morier-TeissierE Delain
Aug 21, 1985·Biochimica Et Biophysica Acta·M Leng
Mar 2, 1993·Biochemistry·Z ReichA Minsky

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