PMID: 8995501Jan 1, 1996Paper

Electropermeabilization in bladder cancer chemotherapy

Cancer Chemotherapy and Pharmacology
Y KubotaM Watanabe

Abstract

Electropermeabilization has been used for the introduction of genes into cells. Using this technique, we introduced the cytotoxic drug bleomycin (BLM) into cells and examined whether the technique might be useful for the treatment of bladder cancer. For electropermeabilization in vitro, we used YTS-1 cells, a human transitional cell carcinoma line. Aliquots of cell suspension were mixed with a solution of BLM and immediately exposed to electric pulses. A high-power pulse generator was used to supply square-shaped pulses of 1250 V/cm (100 micros, eight pulses). After a 2-h post-shock incubation, cells were washed and incubated for one further hour. Then the concentration of BLM in the cells was measured using a bioassay. For electropermeabilization of tissue, we used normal male Wistar rats. The bladder was exposed and 10 mg/kg BLM was injected into the caudal vein. A series of eight pulses with a time constant of 100 micros at an electric field intensity of 1000 V/cm was applied. The bladder, liver and lungs were extracted 1 h later and prepared for quantification of the BLM concentration using the bioassay. Electrotreated cells contained significantly higher concentrations of BLM than nonelectrotreated cells. The concentration...Continue Reading

Citations

Apr 28, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K K F ChengM P Yuen
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K K ChengS S Cheung
Aug 8, 2014·BioMed Research International·Yan BagloOdrun A Gederaas
Mar 23, 2011·The Journal of Urology·Hayato YamamotoShigeru Tsuboi
Jun 30, 2011·The EMBO Journal·Shigeru TsuboiChikara Ohyama
Sep 29, 1998·The Journal of Urology·Y KubotaN Aoyama
Sep 24, 2014·The Journal of Urology·Juan Luis VásquezJulie Gehl
Aug 14, 2020·Cancers·Aleksander KiełbikJulita Kulbacka
Nov 1, 2017·Oncology Letters·Misaki SatoShigeru Tsuboi

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