Nov 3, 1986

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

Nature
C W PikielnyM Rosbash

Abstract

Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

  • References28
  • Citations111

Mentioned in this Paper

Biochemical Pathway
Small Nuclear RNA
Nucleic Acid Precursors
Primary RNA Transcript
SDS-PAGE
Complex (molecular entity)
Nuclear mRNA Cis Splicing, via Spliceosome
RNA Splicing
Poly(A) Tail
RNA, Messenger, Splicing

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