Electrophoretic affinity measurements on microchip. Determination of binding affinities between diketopiperazine receptors and peptide ligands

Electrophoresis
Alexandra R StettlerMaria A Schwarz

Abstract

ACE on a microchip (MC-ACE) is introduced as a fast and reliable method to determine binding affinities. It is based on monitoring the change in the ionic mobility of a receptor upon binding to a ligand, or vice versa. The method is complementary to other standard methods for binding affinity determinations, like isothermal titration calorimetry (ITC), NMR, UV-Vis spectroscopy, etc. and allows for affinity studies of weak to strong binding interactions. The method is attractive since it principally allows for the analysis of the binding affinity of multiple receptors to a given ligand and requires comparatively small quantities of the binding partners (particularly in comparison to affinity measurements on capillary). We demonstrate the applicability of MC-ACE for the determination of the binding affinities between acid-rich diketopiperazine receptors and basic tripeptides in aqueous solution.

References

Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·Niels H H HeegaardN A Guzman
Sep 4, 2001·Chemistry : a European Journal·H WennemersP Krattiger
Apr 13, 2002·The Journal of Organic Chemistry·Matteo Conza, Helma Wennemers
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Ali Akbar SabouryGholam Hosain Hakimelahi
May 13, 2003·Chemical Communications : Chem Comm·Matteo Conza, Helma Wennemers
Feb 11, 2005·Journal of Chromatography. a·Alexandra R Stettler, Maria A Schwarz
Nov 30, 2005·Electrophoresis·Christian Schou, Niels H H Heegaard

Citations

Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Aug 30, 2008·Journal of Molecular Recognition : JMR·Sasa Bjelić, Ilian Jelesarov
Dec 2, 2009·Electrophoresis·Václav Kašička
Dec 30, 2009·Electrophoresis·Chunxia Jiang, D W Armstrong
May 20, 2015·Journal of Separation Science·Sille Štěpánová, Václav Kašička

Related Concepts

Ligands
Oligopeptides
Piperazines
Plasma Protein Binding Capacity
Electrophoresis, Microchip
Diketopiperazine Ion (1-)

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