PMID: 6166731Jul 1, 1981Paper

Electrophoretic analysis of axonally transported proteins in toad retinal ganglion cells

Journal of Neurochemistry
J H Skene, M Willard

Abstract

As a preliminary step to studying changes in axonal transport in regenerating neurons, we have analyzed the composition and organization of polypeptides normally axonally transported in a neuronal system capable of regeneration, i.e., the retinal ganglion cells of the toad, Bufo marinus. We labeled proteins synthesized in the retina with 35S-methionine and subsequently used one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis to analyze labeled, transported proteins in tissues containing segments of the axons (the optic nerve, optic tract, and optic tecta) of the retinal ganglion cells. The transported polypeptides could be divided into five groups according to their apparent transport velocities. Many of the polypeptides of each group were electrophoretically similar to polypeptides of corresponding groups previously described in rabbit and guinea pig retinal ganglion cells, and in some cases, additional properties of the polypeptides indicated that the transported materials of the two vertebrate classes were homologous. These results serve two purposes. First they establish the retinal ganglion cells of the toad Bufo marinus as a model system in which changes in gene expression related to regeneration may...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T Lorenz, M Willard
Oct 1, 1978·The Journal of Cell Biology·M Estridge, R Bunge
Jun 1, 1979·The Journal of Cell Biology·M WillardP Skene
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Nov 1, 1969·Brain Research·G Lázár, G Székely
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M WillardP R Vagelos
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 1, 1964·International Review of Neurobiology·C D CLEMENTE

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Citations

Dec 14, 1999·Molecular Neurobiology·L H AartsP Schotman
Dec 16, 1982·Brain Research·R J Wenthold, M L McGarvey
Jan 1, 1987·Journal of Neurochemistry·D C Larrivee, B Grafstein
Dec 1, 1987·Journal of Neurochemistry·D C Larrivee, B Grafstein
Sep 23, 2014·Neuroscience·A DeFrancesco-LisowitzR E Zigmond
May 1, 1985·Analytical Chemistry·F A BencsathF H Field
May 22, 1995·The Journal of Comparative Neurology·C Duc, S Catsicas

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