Electrophoretic analysis of the "cross-class" interaction between novel inhibitory serpin, squamous cell carcinoma antigen-1 and cysteine proteinases

Electrophoresis
S NawataH Kato

Abstract

We investigated the "cross-class" interaction between cysteine proteinases and a novel inhibitory serpin, recombinant squamous cell carcinoma (rSCC) antigen-1, which inhibits a serine proteinase, chymotrypsin. rSCC antigen-1 inhibited the cysteine proteinases, papain, papaya proteinase IV and cathepsin L. Interestingly, although rSCC antigen-1 formed sodium dodecyl sulfate (SDS)- and heat-stable complexes with chymotrypsin, rSCC antigen-1 gave the 40 kDa fragment and small molecular mass peptide by incubation with papain without forming an SDS- and heat-stable complex. The cleavage was observed between the Gly353-Ser354 bond, indicating that rSCC antigen-1 interacts with cysteine proteinases not at the predicted reactive site P1-P1' portion (Ser354-Ser355), but at the Gly353-Ser354 of the P2-P1 portion. These findings promote understanding of the "suicide inhibition" mechanism of SCC antigen-1 against cysteine proteinases.

References

Sep 1, 1978·Journal of Biochemistry·T TowatariN Katunuma
Jan 1, 1992·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·N TakeshimaH Kato
Mar 1, 1992·European Journal of Biochemistry·W Bode, R Huber
Mar 12, 1990·FEBS Letters·D J ButtleA J Barrett
Nov 27, 1991·Biochemical and Biophysical Research Communications·Y SuminamiH Kato
Jan 1, 1990·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·N TakeshimaH Kato
Jan 29, 1990·FEBS Letters·D J ButtleA J Barrett
Sep 6, 1968·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen
Jan 1, 1981·Methods in Enzymology·A J Barrett, H Kirschke
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·S S SchneiderG A Silverman
Jan 1, 1994·Seminars in Thrombosis and Hemostasis·P A PatstonM Schapira
Jan 4, 1993·FEBS Letters·E Remold-O'Donnell
Apr 1, 1993·FEMS Microbiology Letters·M A CurtisP A Pemberton

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Citations

Jun 19, 2001·Biochimica Et Biophysica Acta·Y SuminamiH Kato
Feb 5, 1999·Biochimica Et Biophysica Acta·Y SakaguchiH Kato
Nov 11, 2005·The FEBS Journal·John T Christeller
Mar 1, 2005·Biochimica Et Biophysica Acta·Yoshinori SuminamiHiroshi Kato
Jun 5, 2001·The Journal of Biological Chemistry·S De FalcoG Fassina
Feb 24, 2017·Surgery Today·Haruhiko Nakamura, Toshihide Nishimura

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