Electrophoretic and antigenic characterisation of Dermatophilus congolensis extracellular products

Veterinary Microbiology
Nicholas C AmbroseR Boid

Abstract

Dermatophilus congolensis is the causative agent of bovine dermatophilosis and lumpy wool in sheep. Two field isolates of D. congolensis, one each from a cow in Ghana and a sheep in Scotland, were cultured for 24-72 h in a synthetic medium based on RPMI-1640. Culture filtrates were examined by SDS-PAGE and considered to contain extracellular products released by growing hyphae and filaments. Electrophoretic profiles of culture filtrates of the two isolates contained common bands and bands that were unique to each isolate. The composition of extracellular products altered with increasing culture periods indicating that specific products were released at different stages of growth. Culture filtrate prepared in the presence of serine protease and metalloprotease inhibitors contained more and better defined bands than that prepared without protease inhibitors indicating the presence of proteases in culture filtrates. Western blot analysis of extracellular products using a panel of sera showed that the two isolates from different host species and distant geographical locations contained cross-reactive antigens. Natural and experimental infections stimulated antibody responses to antigens in culture filtrates, sera from animals that ...Continue Reading

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Citations

Mar 17, 2004·Veterinary Microbiology·Alfredo García-SánchezJavier Hermoso-de-Mendoza
Jun 23, 1999·Parasitology Today·N AmbroseJ C Maillard
May 22, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J LarrasaJ Hermoso de Mendoza
Dec 7, 2007·Veterinary Microbiology·B J NorrisT J Dixon
Feb 11, 2004·FEMS Microbiology Letters·Alfredo Garcia-SanchezJavier Hermoso-de-Mendoza
Dec 22, 2017·Veterinary World·M Ananda ChitraB S M Ronald

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