PMID: 2504932Jul 1, 1989Paper

Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved

Journal of Molecular Evolution
B L Randolph-AndersonJ E Boynton

Abstract

Antibodies to individual chloroplast ribosomal (r-)proteins of Chlamydomonas reinhardtii synthesized in either the chloroplast or the cytoplasm were used to examine the relatedness of Chlamydomonas r-proteins to r-proteins from the spinach (Spinacia oleracea) chloroplast, Escherichia coli, and the cyanobacterium Anabaena 7120. In addition, 35S-labeled chloroplast r-proteins from large and small subunits of C. reinhardtii were co-electrophoresed on 2-D gels with unlabeled r-proteins from similar subunits of spinach chloroplasts, E. coli, and Anabaena to compare their size and net charge. Comigrating protein pairs were not always immunologically related, whereas immunologically related r-protein pairs often did not comigrate but differed only slightly in charge and molecular weight. In contrast, when 35S-labeled chloroplast r-proteins from large and small subunits of a closely related species C. smithii were coelectrophoresed with unlabeled C. reinhardtii chloroplast r-proteins, only one pair of proteins from each subunit showed a net displacement in mobility. Analysis of immunoblots of one-dimensional SDS and two-dimensional urea/SDS gels of large and small subunit r-proteins from these species revealed more antigenic conservati...Continue Reading

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Citations

Jun 14, 2005·Plant Molecular Biology·Ken-Ichi KuchoMasahiro Ishiura
Sep 7, 2005·Photosynthesis Research·María Verónica BeligniStephen P Mayfield
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·James Uniacke, William Zerges
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marco SchottkowskiWilliam Zerges
Dec 7, 2007·The Plant Cell·James Uniacke, William Zerges
Feb 15, 2005·Plant Physiology and Biochemistry : PPB·Einar J Stauber, Michael Hippler
Jun 17, 2003·Journal of Applied Microbiology·S B Young, P Setlow
Apr 14, 2004·Journal of Applied Microbiology·S B Young, P Setlow
Jul 4, 2003·Science Progress·A D Russell
Oct 10, 2015·Journal of Cell Science·Yu ZhanWilliam Zerges

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