Electrophoretic characterization of pyrimidine 5'-nucleotidase of human erythrocytes and its distinction from acid phosphatase

Clinica Chimica Acta; International Journal of Clinical Chemistry
R RosaJ Rosa

Abstract

Electrophoresis of red cell pyrimidine 5'-nucleotidase was carried out on cellulose acetate strips. One major band of activity was found in preparations from human erythrocytes. This enzyme showed a specificity for the pyrimidine nucleotides UMP and CMP. No activity was detected with AMP. Pyrimidine 5'-nucleotidase activity could be separated from that of acid phosphatase with the use of alpha-glycerophosphate. This method may be useful in the study of pyrimidine 5'-nucleotidase deficiency in red cells.

Citations

Dec 8, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y IshidaA Kibe
May 30, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A HironoS Miwa

Related Concepts

Acid Phosphatase
Electrophoresis, Cellulose Acetate
Erythrocytes
Umbilical Cord Blood
Nucleotidases
Pyrimidine Nucleotides

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