Electrophoretic, chromatographic and immunological studies of human urinary proteins

Electrophoresis
M R BüelerD J Vonderschmitt

Abstract

Urinary proteins from both sexes were analyzed by high resolution two-dimensional gel electrophoresis (2-DE). For well reproducible 2-DE patterns, the samples were concentrated and desalted in one step by vacuum dialysis. A reference map for urine proteins was established by the analysis of urine from 10 healthy persons. Proteins in urine that share immunogenicity with serum proteins were identified by use of antibody to whole human-serum protein in an affinity-column fractionation of urine and differential analysis of the adsorbed (serum component) and unadsorbed (non-serum component) fractions. For identification of individual proteins, coelectrophoresis, immunoblotting and affinity chromatography with corresponding antibodies were used. Proteins identified in the map, besides known serum proteins, included: the subunit of Tamm-Horsefall protein, the secretory component of IgA, constant breakdown products of alpha 1-antitrypsin and retinol-binding protein, the five isoforms of the beta chain of human chorionic gonadotropin and the subunit of prostatic acid phosphatase. In addition, we could demonstrate three proteins which are markedly pronounced in female urine, especially pregnant women. To get more information about the na...Continue Reading

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Citations

May 7, 1998·Journal of Biomedical Materials Research·M A WassallS P Denyer
Sep 7, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Milan Chromek, Annelie Brauner
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Apr 22, 2008·Applied and Environmental Microbiology·Rebecca Munk Vejborg, Per Klemm
Nov 14, 2013·International Journal of Proteomics·Holger HusiJames A Ross
Dec 17, 2009·Journal of Proteomics·Giovanni CandianoGian Marco Ghiggeri
Jul 10, 2008·Proteomics. Clinical Applications·Joshua J CoonPhilippe Schmitt-Kopplin
Aug 16, 2014·Journal of Proteomics·Laura SantucciGian Marco Ghiggeri
Sep 1, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·José M González-BuitragoIsabel Lorenzo
May 10, 2005·The Journal of Urology·J N'DowJ P Pearson
Nov 17, 2006·American Journal of Physiology. Renal Physiology·Michael G JanechJohn M Arthur
Oct 9, 2009·Expert Review of Proteomics·Steve GoodisonVirginia Urquidi
Mar 18, 2016·International Journal of Oncology·Holger HusiJames A Ross

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