Electrophoretic patterns of gamma-glutamyltransferase activity eluted from liver tissue

Clinica Chimica Acta; International Journal of Clinical Chemistry
Z O Echetebu, D W Moss

Abstract

Up to three zones of gamma-glutamyltransferase activity were present in 89 samples of human serum after agarose-gel electrophoresis at pH 8.6. Their mobilities relative to albumin were zero, 0.3--0.5, and 0.7--0.9. Incubation of human liver tissue in serum increased the activity of the zones with zero and 0.7--0.9 mobilities, and transiently, of the zone of intermediate mobility. More prolonged incubation caused the intermediate zone to decline, and produced new zones of mobility greater than that of albumin which were not seen in native sera. The mobility of partially-purified liver gamma-glutamyltransferase incubated in serum or protein-free solutions was 0.7--0.8. The intermediate zone was not produced when liver tissue was incubated in protein-free solutions, nor with the purified enzyme in serum or protein-free solutions. The possible relevance of these observations to the electrophoretic patterns of gamma-glutamyltransferase in pathological sera is discussed.

References

Mar 15, 1973·Klinische Wochenschrift·E B IgartuaJ Findor

Citations

Mar 19, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·N E Huseby
Aug 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·P R WenhamA F Smith
Nov 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·P R WenhamA F Smith
Mar 1, 1990·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·I D Mandel
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Celia CarrilloSara R Alonso-Torre
Jul 31, 1980·The New England Journal of Medicine·G CamussiC Tetta
Jan 1, 1980·CRC Critical Reviews in Clinical Laboratory Sciences·D M Goldberg

Related Concepts

Electrophoresis, Agar Gel
Gamma-glutamyl transferase
Liver

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