PMID: 6317487Dec 1, 1983Paper

Electrophoretic separation of prolactin secreted from mouse pituitary glands in vitro

Molecular and Cellular Endocrinology
H DohiY Shoda

Abstract

Prolactin secreted from the mouse pituitary gland in organ culture was characterized after disc or SDS-polyacrylamide gel electrophoresis. Some pituitary glands were cultured with 3H-labelled leucine for 24 h to obtain radioactive prolactin. In disc electrophoresis, immunoreactive, receptor-bindable and 3H-incorporated prolactins formed a single band with the same relative mobility (0.5). Prolactin migrating at Rf = 0.5 was extracted and re-analysed by SDS electrophoresis. A single stained and radioactive band was observed at the same position with an apparent molecular weight of 23 000 regardless of denaturing in the presence of absence of dithiothreitol. No other band was detected. These results indicate that mouse prolactin synthesized and secreted in organ culture is homogeneous and that mouse prolactin is a single-chain molecule.

References

Jan 30, 1979·Biochemical and Biophysical Research Communications·Y N Sinha, S R Baxter
Dec 1, 1978·Molecular and Cellular Endocrinology·S SakaiM R Banerjee
Dec 1, 1977·Endocrinology·G A EvansM G Rosenfeld
Sep 1, 1974·The Journal of Endocrinology·R Yanai, H Nagasawa
Jul 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·K Komoto, H A Bern
Oct 1, 1969·Endocrinology·E V CheeverU J Lewis
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P ColosiF Talamantes
Aug 29, 1980·Biochemical and Biophysical Research Communications·I Mittra

Related Concepts

Sputolysin
SDS-PAGE
Organ Culture Techniques
Pituitary Stalk
Prolactin
Radioimmunoassay
Radioligand Assay
Hormone Receptors, Cell Surface
Prolactin Receptor

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