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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

Proceedings of the National Academy of Sciences of the United States of America

Sep 1, 1979

Harry TowbinJohn R Gordon

PMID: 388439

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Abstract

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained ...read more

Mentioned in this Paper

Peroxidases
Ligands
Antigen-Antibody Reactions
Polyacrylamide Gels
Autoradiography
Urea
Sulfite Cellulose
Ribosomal Proteins
SDS-PAGE
Antigens
161
1
3081
5
Paper Details
References
  • References20
  • Citations26888
12
  • References20
  • Citations26888
12345...

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

Proceedings of the National Academy of Sciences of the United States of America

Sep 1, 1979

Harry TowbinJohn R Gordon

PMID: 388439

DOI:

Abstract

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained ...read more

Mentioned in this Paper

Peroxidases
Ligands
Antigen-Antibody Reactions
Polyacrylamide Gels
Autoradiography
161
1
3081
5

Related Papers

Paper Details
References
  • References20
  • Citations26888
12
  • References20
  • Citations26888
12345...

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