[Electrophoretographic analysis of blood-brain barrier function].

Neurologia Medico-chirurgica
T Uchizawa

Abstract

Intracerebral serum proteins were measured in rats with altered blood-brain barrier (BBB) function. The rats were divided into seven groups of five to 10 each and subjected to the following procedures: controls (NOR); bilateral common carotid artery ligation (BLCL), evaluated after 6 hours; three-vessel occlusion (bilateral common carotid and right vertebral arteries) (TVO), evaluated after 6 hours; cold injury (BE6 and BE24), evaluated after 6 and 24 hours; and intracarotid injection of a hypertonic solution (BBB6 and BBB24), evaluated after 6 and 24 hours. The protein components of concentrated, water-soluble extracts of each rat's brain were analyzed by cellulose acetate membrane electrophoresis. Serum proteins of each rat were also analyzed. The intracerebral albumin fraction was increased in groups BE24, BBB6, and some TVO rats. The intracerebral alpha-1 fraction was increased in group BBB24. No difference was found in the electrophoretographic patterns of groups NOR and BLCL. These findings demonstrate that serum albumin is increased in TVO, and suggest that the BBB is damaged as in cold injury, which is thought to cause edema. The predominant intracerebral serum protein appeared to change from the alpha-1 fraction to the...Continue Reading

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