Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract

Chemical Senses
J Du, R M Bradley

Abstract

The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.

Citations

Jul 23, 1998·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B M WethertonL Schweitzer
Aug 30, 2018·The Journal of Comparative Neurology·Carlos Escobar VásquezAlberto A Rasia-Filho
Oct 5, 2002·Journal of Neurophysiology·Arturas GrigaliunasCharlotte M Mistretta
Nov 3, 2017·Journal of Neurophysiology·Alison BoxwellJoseph B Travers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.