Electrophysiological and Morphological Characterization of Chrna2 Cells in the Subiculum and CA1 of the Hippocampus: An Optogenetic Investigation

Frontiers in Cellular Neuroscience
Heather NicholSylvain Williams

Abstract

The nicotinic acetylcholine receptor alpha2 subunit (Chrna2) is a specific marker for oriens lacunosum-moleculare (OLM) interneurons in the dorsal CA1 region of the hippocampus. It was recently shown using a Chrna2-cre mice line that OLM interneurons can modulate entorhinal cortex and CA3 inputs and may therefore have an important role in gating, encoding, and recall of memory. In this study, we have used a combination of electrophysiology and optogenetics using Chrna2-cre mice to determine the role of Chrna2 interneurons in the subiculum area, the main output region of the hippocampus. We aimed to assess the similarities between Chrna2 subiculum and CA1 neurons in terms of the expression of interneuron markers, their membrane properties, and their inhibitory input to pyramidal neurons. We found that subiculum and CA1 dorsal Chrna2 cells similarly expressed the marker somatostatin and had comparable membrane and firing properties. The somas of Chrna2 cells in both regions were found in the deepest layer with axons projecting superficially. However, subiculum Chrna2 cells displayed more extensive projections with dendrites which occupied a significantly larger area than in CA1. The post-synaptic responses elicited by Chrna2 cell...Continue Reading

References

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Citations

Aug 8, 2018·Cell and Tissue Research·Sam A Booker, Imre Vida
Oct 15, 2019·ELife·Zoé Christenson WickEsther Krook-Magnuson
May 20, 2021·Trends in Neurosciences·Klas Kullander, Lisa Topolnik
Aug 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·James DelormeSara J Aton

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Methods Mentioned

BETA
transgenic

Key Resources (RRID) Mentioned

IMSR_JAX
AB_2195930
AB_141708
SCR_003070
SCR_002448
SCR_012730
AB_523903
AB_2209751
AB_142672
AB_143011

Software Mentioned

ImageJ
pClamp10
Neurolucida
VassarStats
Stereo Investigator 13
SPSS
Clampfit10

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