Electrophysiological characteristic of corticoaccumbens synapses in rat mesolimbic system reconstructed using organotypic slice cultures

Brain Research
Takehiko MaedaShiroh Kishioka

Abstract

The nucleus accumbens (NAc) is a component of the mesolimbic system involved in drug dependence. Activity of nucleus accumbens neurons is modulated by glutamatergic afferents from the prefrontal cortex and by dopaminergic afferents from the ventral tegmental area (VTA). In the present study, we reconstructed the mesolimbic system using organotypic slice cultures and examined the effects of dopaminergic agents on synaptic activity in the prefrontal cortex-nucleus accumbens synapses. A slice of each of the prefrontal cortex, nucleus accumbens and ventral tegmental area in newborn rat, was arranged on a multi-electrode dish (MED) filled with culture medium so that they contacted each other, termed a 'triple culture'. Extracellular recording using microelectrodes on the multi-electrode dish showed that a single electrical stimulation of the prefrontal cortex slice evoked field excitatory postsynaptic potential, and that population spikes occurred spontaneously in the nucleus accumbens area of the triple culture. The amplitude of evoked field excitatory postsynaptic potentials and the frequency of spontaneous population spikes were decreased by glutamatergic antagonists, D(-)-2-amino-5-phosphonovaleric acid and 6-cyano-7-nitroquinox...Continue Reading

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Citations

Jun 19, 2013·Nature Communications·Dario BesussoLiliana Minichiello
Jul 8, 2008·Journal of Pharmacological and Toxicological Methods·William S RedfernJean-Pierre Valentin
Feb 19, 2009·Journal of Neuroscience Research·Kohei HommaMasayo Takahashi
Oct 20, 2010·Neuroscience and Biobehavioral Reviews·Danielle S Stolzenberg, Michael Numan
Nov 14, 2006·Behavioral and Cognitive Neuroscience Reviews·Michael Numan

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