Electrophysiological characterization of BmK M1, an alpha-like toxin from Buthus martensi Karsch venom

FEBS Letters
Cyril GoudetJan Tytgat

Abstract

The present study investigates the electrophysiological actions of BmK M1, an alpha-like toxin purified from the venom of the scorpion Buthus martensi Karsch, on voltage-gated Na+ channels. Using the voltage clamp technique, we assessed the BmK M1 activity on the cardiac Na+ channel (hH1) functionally expressed in Xenopus oocytes. The main actions of the toxin are a concentration-dependent slowing of the inactivation process and a hyperpolarizing shift of the steady-state inactivation. This work is the first electrophysiological characterization of BmK M1 on a cloned Na+ channel, demonstrating that this toxin belongs to the class of scorpion alpha-toxins. Our results also show that BmK M1 can be considered as a cardiotoxin.

References

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Citations

Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Cyril GoudetJan Tytgat
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Andrei KondratievGordon F Tomaselli
Oct 5, 2011·Molecular & Cellular Proteomics : MCP·Shunyi ZhuJan Tytgat
Jul 22, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xing-Hua FengYong-Hua Ji
Mar 27, 2007·Journal of Biochemical and Biophysical Methods·Mingxin MaSiling Wang
Nov 8, 2006·Toxicon : Official Journal of the International Society on Toxinology·Frank Bosmans, Jan Tytgat
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Hui LiuJan Tytgat
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·T M MendesE Kalapothakis
Oct 30, 2016·Neuropharmacology·David M HousleyErnest A Jennings

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