Electrophysiological characterization of Na+ currents in acutely isolated human hippocampal dentate granule cells

The Journal of Physiology
G ReckziegelB W Urban

Abstract

1. Properties of voltage-dependent Na+ currents were investigated in forty-two dentate granule cells (DGCs) acutely isolated from the resected hippocampus of twenty patients with therapy-refractory temporal lobe epilepsy (TLE) using the whole-cell patch-clamp technique. 2. Depolarizing voltage commands elicited large, rapidly activating and inactivating Na+ currents (140 pS microm-2; 163 mM extracellular Na+) that were reduced in amplitude by lowering the Na+ gradient (43 mM extracellular Na+). At low temperatures (8-12 C), the time course of Na+ currents slowed and could be well described by the model of Hodgkin & Huxley. 3. Na+ currents were reversibly blocked by tetrodotoxin (TTX) and saxitoxin (STX) with a half-maximal block of 4.7 and 2.6 nM, respectively. In order to reduce series resistance errors, the Na+ current was partially blocked by low toxin concentrations (10-15 nM) in the experiments described below. Under these conditions, Na+ currents showed a threshold of activation of about -50 mV, and the voltages of half-maximal activation and inactivation were -29 and -55 mV, respectively. 4. The time course of recovery from inactivation could be described with a double-exponential function (time constants, 3-20 and 60-20...Continue Reading

References

Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
Jan 1, 1992·Methods in Enzymology·C M Armstrong, W F Gilly
Oct 1, 1991·Physiological Reviews·J Patlak
Apr 1, 1991·The Journal of Membrane Biology·P H Barry, J W Lynch
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B Dargent, F Couraud
Feb 1, 1987·The Journal of General Physiology·T Gonoi, B Hille
Mar 1, 1988·The Journal of General Physiology·P SahP W Gage
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1983·Annual Review of Biophysics and Bioengineering·R J French, R Horn
Sep 1, 1993·Journal of Neurophysiology·A ScholzH Bostock
Aug 1, 1994·Pflügers Archiv : European journal of physiology·M SchneiderR Rüdel
Mar 1, 1994·Journal of Neurophysiology·T R CumminsG G Haddad
Sep 1, 1993·Journal of Neuropathology and Experimental Neurology·H K WolfO D Wiestler
Jan 1, 1996·Brain Research. Molecular Brain Research·A J LombardoG B Brown
Jan 1, 1995·Epilepsia·R L Macdonald, K M Kelly
Jan 1, 1997·The Journal of Physiology·H BeckC E Elger

❮ Previous
Next ❯

Citations

Sep 5, 1998·Brain Research. Molecular Brain Research·C FrenkelB W Urban
Nov 9, 2002·Epilepsia·Rüdiger Köhling
Nov 26, 2005·Progress in Neurobiology·Massimo AvoliRüdiger Köhling
Jun 28, 2002·Neuron·Christoph LossinAlfred L George
Nov 19, 2003·The European Journal of Neuroscience·Andrea StraessleJean-Marc Fritschy
Aug 23, 2019·PLoS Computational Biology·Geir HalnesKjetil Hodne
Apr 18, 2002·Brain Pathology·Douglas A CoulterWolfgang Löscher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.