Electrophysiological classification of P2X7 receptors in rat cultured neocortical astroglia.

British Journal of Pharmacology
W NörenbergP Illes

Abstract

P2X7 receptors are ATP-gated cation channels mediating important functions in microglial cells, such as the release of cytokines and phagocytosis. Electrophysiological evidence that these receptors also occur in CNS astroglia is rare and rather incomplete. We used whole-cell patch-clamp recordings to search for P2X7 receptors in astroglial-neuronal co-cultures prepared from the cerebral cortex of rats. All the astroglial cells investigated responded to ATP with membrane currents, reversing around 0 mV. These currents could be also detected in isolated outside-out patch vesicles. The results of the experiments with the P2X [alpha,beta-methylene ATP and 2'-3'-O-(4-benzoyl) ATP] and P2Y receptor agonists [adenosine 5'-O-(2-thiodiphosphate), uridine 5'-diphosphate, uridine 5'-triphosphate (UTP) and UDP-glucose] suggested the involvement of P2X receptors in this response. The potentiation of ATP responses in a low divalent cation or alkaline bath, but not by ivermectin, made it likely that a P2X7 receptor is operational. Blockade of the ATP effect by the P2X7 antagonists Brilliant Blue G, calmidazolium and oxidized ATP corroborated this assumption. Rat cultured cortical astroglia possesses functional P2X7 receptors. It is suggested ...Continue Reading

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Citations

May 1, 2012·Purinergic Signalling·Heike FrankePeter Illes
Jun 26, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Patrizia RubiniPeter Illes
Sep 17, 2014·Trends in Pharmacological Sciences·Beáta Sperlágh, Peter Illes
Apr 6, 2012·Neurochemical Research·Alexei VerkhratskyVladimir Parpura
Feb 16, 2011·Seminars in Cell & Developmental Biology·Ulyana LaloYuri Pankratov
Mar 8, 2011·Seminars in Cell & Developmental Biology·Arthur M Butt
Apr 18, 2012·British Journal of Pharmacology·W NörenbergM Schaefer
Jan 1, 2012·Wiley Interdisciplinary Reviews. Membrane Transport and Signaling·Alexei VerkhratskyMaiken Nedergaard
Sep 17, 2013·Journal of Cellular Physiology·Adolfo E CuadraJosé R Lemos
Aug 11, 2015·Neuropharmacology·Peter Illes, Alexei Verkhratsky
Jun 4, 2013·Neuropharmacology·Nanette MessemerPatrizia Rubini
Jun 19, 2013·Neuropharmacology·Ukpong B EyoMichael E Dailey
Nov 14, 2012·Neurochemistry International·Anna LeichsenringPeter Illes
Oct 10, 2013·Neuroscience Letters·Heike Franke, Peter Illes
Mar 16, 2012·Neurochemistry International·Chiara CervettoManuela Marcoli
May 18, 2016·The Journal of General Physiology·Wolfgang NörenbergMichael Schaefer
Jan 30, 2019·Expert Opinion on Therapeutic Targets·Peter IllesYong Tang
Oct 9, 2013·The Journal of Pharmacology and Experimental Therapeutics·Elvira SalasEsmerilda G Delicado
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Aug 24, 2019·Frontiers in Molecular Neuroscience·Robin KoppAnnette Nicke
Oct 21, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Peter IllesHeike Franke

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