PMID: 2506759Sep 1, 1989Paper

Electrophysiological effects of mucosal Cl- removal in Necturus gallbladder epithelium

The American Journal of Physiology
J S Stoddard, L Reuss

Abstract

The factors responsible for the cell membrane hyperpolarization elicited in Necturus gallbladder epithelium on Cl- removal from the mucosal bathing solution were evaluated with conventional and ion-sensitive microelectrode techniques. Cl- removal causes reversal of apical Cl- -HCO3- exchange, resulting in a fall in intracellular Cl- activity (aiCl) and an increase in intracellular pH (pHi). Concomitantly, the cell membranes hyperpolarize to values close to the K+ equilibrium potential (EK), aiNa falls, and aiK rises. The observed changes in membrane voltage are not attributable to a pHi-dependent increase in cell membrane K+ permeability (PK), because 1) the cell membrane resistances increased and 2) elevating solution partial pressure of CO2 (PCO2) to counterbalance the cellular alkalinization on mucosal Cl- removal caused a further hyperpolarization of the cell membranes to values greater than EK. This additional hyperpolarization was related to the activity of the Na+ pump, inasmuch as it was accompanied by an increase in aiNa and was ouabain sensitive. These results are consistent with, but do not prove, pump electrogenicity. During the period of Cl- removal from the mucosal bathing solution, the cell membrane depolarizatio...Continue Reading

References

Jun 7, 1979·The Journal of Membrane Biology·J Graf, G Giebisch
Dec 4, 1975·The Journal of Membrane Biology·C H van Os, J F Slegers
Jan 1, 1987·Annual Review of Physiology·L Reuss, J S Stoddard
May 1, 1988·The Journal of Membrane Biology·J S Stoddard, L Reuss
Jan 1, 1972·The Journal of Membrane Biology·E Frömter
May 1, 1983·The Journal of General Physiology·K U Petersen, L Reuss
Sep 1, 1983·Biophysical Journal·J F García-DíazA Essig
Jan 1, 1984·The Journal of General Physiology·S A Weinman, L Reuss
May 1, 1980·The Journal of Physiology·W Nagel
Jan 1, 1983·The Journal of Membrane Biology·J Fernando Garcia-DiazW M Armstrong
Oct 1, 1982·Pflügers Archiv : European journal of physiology·K SuzukiE Frömter
Sep 1, 1964·The Journal of General Physiology·J M DIAMOND

❮ Previous
Next ❯

Citations

Mar 13, 2012·Journal of Cardiovascular Pharmacology·Noboru TodaTomio Okamura
May 1, 1990·Pflügers Archiv : European journal of physiology·K U PetersenJ M Winterhager
Jan 1, 1989·Annals of the New York Academy of Sciences·L Reuss
Nov 1, 1992·Pflügers Archiv : European journal of physiology·I NovakE H Larsen
Aug 17, 2001·Developmental Psychobiology·M CohenM M Myers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.