Electrophysiological Evidence for Intrinsic Pacemaker Currents in Crayfish Parasol Cells

PloS One
DeForest Mellon

Abstract

I used sharp intracellular electrodes to record from parasol cells in the semi-isolated crayfish brain to investigate pacemaker currents. Evidence for the presence of the hyperpolarization-activated inward rectifier potassium current was obtained in about half of the parasol cells examined, where strong, prolonged hyperpolarizing currents generated a slowly-rising voltage sag, and a post-hyperpolarization rebound. The amplitudes of both the sag voltage and the depolarizing rebound were dependent upon the strength of the hyperpolarizing current. The voltage sag showed a definite threshold and was non-inactivating. The voltage sag and rebound depolarization evoked by hyperpolarization were blocked by the presence of 5-10 mM Cs2+ ions, 10 mM tetraethyl ammonium chloride, and 10 mM cobalt chloride in the bathing medium, but not by the drug ZD 7288. Cs+ ions in normal saline in some cells caused a slight increase in mean resting potential and a reduction in spontaneous burst frequency. Many of the neurons expressing the hyperpolarization-activated inward potassium current also provided evidence for the presence of the transient potassium current IA, which was inferred from experimental observations of an increased latency of post-hy...Continue Reading

References

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Sep 20, 2005·The Journal of Experimental Biology·DeForest Mellon
Apr 15, 2011·Journal of Neurophysiology·Marie L GoeritzRonald M Harris-Warrick

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Citations

Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Francisco Javier MazaAlejandro Delorenzi
Aug 4, 2019·The Journal of Comparative Neurology·Nicholas J Strausfeld, Marcel E Sayre

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