Electrophysiological interrogation of asymmetric droplet interface bilayers reveals surface-bound alamethicin induces lipid flip-flop

Biochimica Et Biophysica Acta. Biomembranes
Graham J TaylorStephen A Sarles

Abstract

The droplet interface bilayer (DIB) method offers simple control over initial leaflet compositions in model membranes, enabling an experimental path to filling gaps in our knowledge about the interplay between compositional lipid asymmetry, membrane properties, and the behaviors of membrane-active species. Yet, the stability of lipid leaflet asymmetry in DIBs has received very little attention, particularly in the presence of peptides and ion channels that are often studied in DIBs. Herein, we demonstrate for the first time parallel, capacitance-based measurements of intramembrane potential with arrays of asymmetric DIBs assembled in a microfluidic device to characterize the stability of leaflet asymmetry over many hours in the presence and absence of membrane-active peptides. DIBs assembled from opposing monolayers of the ester (DPhPC) and ether (DOPhPC) forms of diphytanoyl-phosphatidylcholine yielded asymmetric bilayers with leaflet compositions that were stable for at least 18 h as indicated by a stable |137 mV| intramembrane potential. In contrast, the addition of surface-bound alamethicin peptides caused a gradual, concentration-dependent decrease in the magnitude of the dipole potential difference. Intermittent current-v...Continue Reading

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Citations

Aug 4, 2020·Applied Biochemistry and Biotechnology·Himadri Gourav Behuria, Santosh Kumar Sahu
Sep 5, 2019·International Journal of Molecular Sciences·Chetna TyagiFerenc Ötvös
May 6, 2019·Experimental Biology and Medicine·Maxwell Allen-BentonPaula J Booth
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Mar 10, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Michelle M Makhoul-Mansour, Eric C Freeman
Apr 8, 2019·Biophysical Journal·Haden L ScottFrancisco N Barrera
Jun 9, 2020·Biochimica Et Biophysica Acta. Biomembranes·R J ClarkeK Cao
Mar 17, 2021·Analytical Chemistry·Simon BachlerPetra S Dittrich
Jun 10, 2021·Chemical Communications : Chem Comm·Elanna B Stephenson, Katherine S Elvira
May 20, 2020·The Journal of Physical Chemistry. B·William T McClinticC Patrick Collier
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Aug 14, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Michael H L NguyenDrew Marquardt
Jan 25, 2022·ACS Applied Materials & Interfaces·Joyce El-BeyrouthyEric C Freeman

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