PMID: 591915Nov 1, 1977

Electrophysiological measurement of the number of rhodopsin molecules in single Limulus photoreceptors

The Journal of General Physiology
J Lisman, H Bering

Abstract

Two partly independent electrophysiological methods are described for measuring the number of rhodopsin molecules (R) in single ventral photoreceptors. Method 1 is based on measurements of the relative intensity required to elicit a quantal response and the relative intensity required to half-saturate the early receptor potential (ERP). Method 2 is based on measurements of the absolute intensity required to elicit a quantal response. Both methods give values of R approximately equal to 10(9). From these and other measurements, estimates are derived for the surface density of rhodopsin (8,000/micrometer2), the charge movement during the ERP per isomerized rhodopsin (20 X 10(-21) C), and the half-time for thermal isomerization of rhodopsin (36yr).

References

Nov 1, 1976·The Journal of General Physiology·J Lisman, Y Sheline
Apr 14, 1977·Nature·W A HarrisW S Stark
Jul 6, 1976·Cell and Tissue Research·E Eguchi, T H Waterman
Jun 1, 1976·The Journal of Cell Biology·H R Fernandez, E E Nickel
Nov 28, 1973·Biochimica Et Biophysica Acta·F J Daemen
May 1, 1973·The Journal of General Physiology·S YEANDLE, J B Spiegler
Jul 1, 1973·The Journal of General Physiology·P HillmanB Minke
Dec 24, 1973·Experimental Eye Research·Y S Chen, W L Hubbell
Mar 1, 1974·The Journal of General Physiology·J E Brown, M I Mote
Jul 1, 1972·The Journal of General Physiology·R Srebro, M Behbehani
Jan 1, 1965·Cold Spring Harbor Symposia on Quantitative Biology·W A Hagins
Apr 5, 1968·Science·F A DodgeJ Toyoda
Sep 1, 1969·The Journal of General Physiology·A W ClarkA Mauro
Sep 1, 1969·The Journal of General Physiology·R Millecchia, A Mauro
Sep 1, 1969·The Journal of General Physiology·R Millecchia, A Mauro
Aug 1, 1956·Journal of the Optical Society of America·H B BARLOW
Apr 16, 1960·Nature·R HUBBARD, G WALD
Jan 1, 1964·The Journal of General Physiology·M G FUORTES, S YEANDLE
Mar 1, 1964·The Journal of General Physiology·T P Williams
Nov 1, 1964·The Journal of General Physiology·A R ADOLPH
Feb 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·R Hubbard, A Kropf

Citations

Jan 1, 1989·Vision Research·S GagnéD G Stavenga
Jan 2, 2009·Nature·Michael Tri H DoKing-Wai Yau
Nov 15, 2011·The Journal of Biological Chemistry·Claudio PunzoConstance L Cepko
Feb 1, 1979·The Journal of General Physiology·J Lisman, J Strong
Nov 1, 1986·The Journal of General Physiology·E C Johnson, W L Pak
Mar 1, 1989·The Journal of General Physiology·P M O'Day, M P Gray-Keller
Jan 1, 1981·Progress in Biophysics and Molecular Biology·G L Fain, J Lisman
Jan 21, 1978·Lancet
Aug 31, 1999·Biophysical Journal·J M Sullivan, P Shukla

Related Concepts

Electric Stimulation Technique
Electrophysiology (Science)
Tachypleus
Isomerism
Resting Potentials
Ciliary Photoreceptor Cells
Quantum Theory
Retinal Pigments
Rhodopsin
Thermodynamics

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