PMID: 9188766Jan 1, 1997Paper

Electrophysiological properties of different cell types in the shark rectal gland

Life Sciences
Y F Xiao

Abstract

Electrophysiological properties of different cell types were studied in single rectal gland cells of Squalus acanthias by the whole-cell voltage clamp technique. Based on electrophysiological characteristics and primary morphological observations (light microscope, X400), three cell types (named as I, II, and III) were found in isolated fresh cells and two cell types (I and II) in primary cultured cells of the shark rectal gland (SRG). Type I cells had both Cl- (I(Cl)) and the inwardly rectifying K+ channel (I(K1)). Type II and III cells only had I(Cl) Under X400 light microscope granular materials in the cytoplasm were found in Type I and II cells, but not in Type III cells. The data from this study show that 65 % of isolated fresh SRG cells strongly expressed the K+ channel with much less amount of the Cl- channel and 35% had only I(Cl). In sharp contrast, 11% had I(K1) and I(Cl), and 89% had only I(Cl) in cultured SRG cells. Extracellular application of 10 microM forskolin significantly enhanced I(Cl) in primary cultured SRG cells. This enhancement was influenced by intracellular Ca2+ and blocked by 50 microM Ni2+. Other compounds, such as vasoactive intestinal peptide (VIP) and 8-(4-chlorophenylthio)-adenosine3':5'-cyclic m...Continue Reading

References

Dec 11, 1991·The Journal of Clinical Investigation·G G KelleyJ N Forrest
Apr 1, 1985·Pflügers Archiv : European journal of physiology·R GregerH Gögelein
Jan 1, 1985·Annals of the New York Academy of Sciences·F H Epstein, P Silva
Jun 1, 1987·Pflügers Archiv : European journal of physiology·H GögeleinR Greger
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·R GregerE Schlatter
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C LondosJ Wolff
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y F XiaoA Leaf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.